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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-GSK3 alpha antibody [EP793Y] (ab224964)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GSK3 alpha antibody [EP793Y] (ab224964)
  • Alexa Fluor® 488 Anti-GSK3 alpha antibody [EP793Y] (ab224964)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EP793Y] to GSK3 alpha
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

You may also be interested in

Primary
Product image
Anti-GSK3 alpha antibody [EP793Y] - BSA and Azide free (ab236006)
Protein
Recombinant human GSK3 alpha protein (ab42597)

View more associated products

Overview

  • Product name

    Alexa Fluor® 488 Anti-GSK3 alpha antibody [EP793Y]
    See all GSK3 alpha primary antibodies
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EP793Y] to GSK3 alpha
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP793Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Cdks
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Heart
    • Apoptosis
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

Associated products

  • Alternative Versions

    • Anti-GSK3 alpha antibody [EP793Y] - BSA and Azide free (ab236006)
    • Anti-GSK3 alpha antibody [EP793Y] (ab40870)
  • Isotype control

    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
  • Recombinant Protein

    • Recombinant human GSK3 alpha protein (ab42597)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab224964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
All applications
Mouse
Rat
Application Abreviews Notes
ICC/IF
1/1000.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

Notes
ICC/IF
1/1000.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

Target

  • Function

    Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession P49840 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2931 Human
    • Entrez Gene: 606496 Mouse
    • Entrez Gene: 50686 Rat
    • Omim: 606784 Human
    • SwissProt: P49840 Human
    • SwissProt: Q2NL51 Mouse
    • SwissProt: P18265 Rat
    • Unigene: 466828 Human
    • Unigene: 294664 Mouse
    • Unigene: 36807 Rat
    see all
  • Alternative names

    • DKFZp686D0638 antibody
    • Glycogen synthase kinase 3 alpha antibody
    • Glycogen synthase kinase-3 alpha antibody
    • GSK 3 alpha antibody
    • GSK 3A antibody
    • GSK-3 alpha antibody
    • Gsk3a antibody
    • GSK3A_HUMAN antibody
    • GSK3alpha antibody
    • Serine/threonine protein kinase GSK3A antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GSK3 alpha antibody [EP793Y] (ab224964)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GSK3 alpha antibody [EP793Y] (ab224964)

    ab224964 staining GSK3 alpha in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab224964 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-GSK3 alpha antibody [EP793Y] (ab224964)
    Alexa Fluor® 488 Anti-GSK3 alpha antibody [EP793Y] (ab224964)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab224964? Please let us know so that we can cite the reference in this datasheet.

ab224964 has not yet been referenced specifically in any publications.

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