Alexa Fluor® 488 Anti-HDAC2 antibody [3F3] (ab208120)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-HDAC2 antibody [3F3] (ab208120)

    Key features and details

    • Alexa Fluor® 488 Mouse monoclonal [3F3] to HDAC2
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
    • Isotype: IgG

    Overview

    • Product name

      Alexa Fluor® 488 Anti-HDAC2 antibody [3F3]
      See all HDAC2 primary antibodies

    • Description

      Alexa Fluor® 488 Mouse monoclonal [3F3] to HDAC2

    • Host species

      Mouse

    • Conjugation

      Alexa Fluor® 488. Ex: 495nm, Em: 519nm

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Chicken, Cow

    • Immunogen

      Synthetic peptide within Human HDAC2 aa 450 to the C-terminus. The exact sequence is proprietary.
      Database link: Q92769

    • Positive control

      • ICC/IF: HeLa cells

    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

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      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

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    Properties

    Applications

    Our Abpromise guarantee covers the use of ab208120 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/10000.

    This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)

    Target

    • Function

      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
      Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.

    • Tissue specificity

      Widely expressed; lower levels in brain and lung.

    • Sequence similarities

      Belongs to the histone deacetylase family. HD type 1 subfamily.

    • Post-translational
      modifications

      S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • D10Wsu179e antibody
      • HD 2 antibody
      • HD2 antibody
      • HDAC 2 antibody
      • Hdac2 antibody
      • HDAC2_HUMAN antibody
      • Histone deacetylase 2 (HD2) antibody
      • Histone deacetylase 2 antibody
      • OTTHUMP00000017046 antibody
      • OTTHUMP00000227077 antibody
      • OTTHUMP00000227078 antibody
      • RPD3 antibody
      • transcriptional regulator homolog RPD3 antibody
      • YAF1 antibody
      • YY1 associated factor 1 antibody
      • YY1 transcription factor binding protein antibody
      • Yy1bp antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-HDAC2 antibody [3F3] (ab208120)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-HDAC2 antibody [3F3] (ab208120)

      ab208120 staining HDAC2 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208120 at 1/10000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    References (0)

    Publishing research using ab208120? Please let us know so that we can cite the reference in this datasheet.

    ab208120 has not yet been referenced specifically in any publications.

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