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Alexa Fluor® 488 Anti-Histone H4 antibody [mAbcam 31830] (ab197513)

Submit a review Submit a question References (1)
Flow Cytometry - Alexa Fluor® 488 Anti-Histone H4 antibody [mAbcam 31830] (ab197513)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H4 antibody [mAbcam 31830] (ab197513)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [mAbcam 31830] to Histone H4
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

Overview

  • Product name

    Alexa Fluor® 488 Anti-Histone H4 antibody [mAbcam 31830]
    See all Histone H4 primary antibodies

  • Description

    Alexa Fluor® 488 Mouse monoclonal [mAbcam 31830] to Histone H4

  • Host species

    Mouse

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab13843)

  • Positive control

    • ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    mAbcam 31830

  • Myeloma

    Sp2/0-Ag14

  • Isotype

    IgG1

  • Light chain type

    kappa

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab197513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).
Flow Cyt
1/500.
Notes
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).
Flow Cyt
1/500.

Target

Images

  • Flow Cytometry - Alexa Fluor® 488 Anti-Histone H4 antibody [mAbcam 31830] (ab197513)
    Flow Cytometry - Alexa Fluor® 488 Anti-Histone H4 antibody [mAbcam 31830] (ab197513)

    Overlay histogram showing HeLa cells stained with ab197513 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197513, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 (monoclonal) Alexa Fluor® 488 (ab171463) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H4 antibody [mAbcam 31830] (ab197513)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H4 antibody [mAbcam 31830] (ab197513)

    ab197513 staining Histone H4 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References (1)

Publishing research using ab197513? Please let us know so that we can cite the reference in this datasheet.

ab197513 has been referenced in 1 publication.

  • Uroda T  et al. Visualizing the functional 3D shape and topography of long noncoding RNAs by single-particle atomic force microscopy and in-solution hydrodynamic techniques. Nat Protoc 15:2107-2139 (2020). PubMed: 32451442

Customer reviews and Q&As

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