RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab185018)

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Flow Cytometry - Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab185018)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab185018)
  • Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab185018)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR5777] to HP1 alpha - Heterochromatin marker
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker
    See all HP1 alpha primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR5777] to HP1 alpha - Heterochromatin marker

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human HP1 alpha aa 150 to the C-terminus.

  • Positive control

    • ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    Alternative versions available:
    Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028) - Knockout validated

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab185018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
Flow Cyt 1/50.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.

Target

  • Function

    Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

  • Sequence similarities

    Contains 2 chromo domains.

  • Post-translational
    modifications

    Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
    Ubiquitinated.

  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
  • Information by UniProt

  • Database links

    • Alternative names

      • Antigen p25 antibody
      • CBX5 antibody
      • CBX5_HUMAN antibody
      • CG8409 antibody
      • Chromobox 5 antibody
      • Chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody
      • Chromobox homolog 5 antibody
      • Chromobox protein homolog 5 antibody
      • Epididymis luminal protein 25 antibody
      • HEL25 antibody
      • Heterochromatin protein 1 alpha antibody
      • Heterochromatin protein 1 antibody
      • Heterochromatin protein 1 homolog alpha antibody
      • HP1 alpha antibody
      • HP1 alpha homolog antibody
      • HP1 antibody
      • HP1A antibody
      • HP1Hs alpha antibody
      • Su(var)205 antibody
      see all

    Images

    • Flow Cytometry - Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab185018)
      Flow Cytometry - Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab185018)

      Overlay histogram showing HeLa cells stained with ab185018 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab185018, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab185018)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab185018)

      ab185018 staining HP1 alpha in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab185018 (Alexa Fluor® 488) at a working dilution of 1 in 50 overnight at +4°C (shown in green). Alexa Fluor® 350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410).

    • Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab185018)
      Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab185018)

    References (1)

    Publishing research using ab185018? Please let us know so that we can cite the reference in this datasheet.

    ab185018 has been referenced in 1 publication.

    • Deng W  et al. CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells. Proc Natl Acad Sci U S A 112:11870-5 (2015). PubMed: 26324940

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