Alexa Fluor® 488 Anti-HSD17B4 antibody [2D3BB5BF10] (ab198591)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-HSD17B4 antibody [2D3BB5BF10] (ab198591)](https://www.abcam.com/ps/products/198/ab198591/Images/ab198591-238977-20141208-ab198591-ap2257964-HepG2p-5ug.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-HSD17B4 antibody [2D3BB5BF10] (ab198591)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [2D3BB5BF10] to HSD17B4
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG2a
Overview
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Product name
Alexa Fluor® 488 Anti-HSD17B4 antibody [2D3BB5BF10]
See all HSD17B4 primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [2D3BB5BF10] to HSD17B4 -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Tissue, cells or virus corresponding to Human HSD17B4. Human liver mitochondria fraction.
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Positive control
- ICC/IF: HeLa and HepG2 cells.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
Clonality
Monoclonal -
Clone number
2D3BB5BF10 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab198591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. This product gave a positive signal in HeLa and HepG2 cells fixed with 4% formaldehyde (10 min) |
Target
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Function
Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. -
Tissue specificity
Present in many tissues with highest concentrations in liver, heart, prostate and testis. -
Pathway
Lipid metabolism; fatty acid beta-oxidation. -
Involvement in disease
D-bifunctional protein deficiency
Perrault syndrome 1 -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 1 MaoC-like domain.
Contains 1 SCP2 domain. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 3295 Human
- Omim: 601860 Human
- SwissProt: P51659 Human
- Unigene: 406861 Human
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Alternative names
- 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody
- 17 beta HSD 4 antibody
- 17 beta HSD IV antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-HSD17B4 antibody [2D3BB5BF10] (ab198591)
ab198591 staining HSD17B4 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198591 at 1/100 dilution (shown in red) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-HSD17B4 antibody [2D3BB5BF10] (ab198591)](https://www.abcam.com/ps/products/198/ab198591/Images/ab198591-238976-20141208-ab198591-ap2257964-HeLap-5ug.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-HSD17B4 antibody [2D3BB5BF10] (ab198591)ab198591 staining HSD17B4 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198591 at 1/100 dilution (shown in red) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
Datasheets and documents
References (0)
ab198591 has not yet been referenced specifically in any publications.
