Recombinant Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234)
![Immunocytochemistry - Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234)](https://www.abcam.com/ps/products/197/ab197234/Images/ab197234-237314-anti-ki67-antibody-epr3610-alexa-fluor-488-immunofluorescence.jpg "Immunocytochemistry - Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR3610] to Ki67
- Suitable for: Flow Cyt (Intra), ICC
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610]
See all Ki67 primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR3610] to Ki67 -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: Flow Cyt (Intra), ICCmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: HeLa cells, HAP1 WT and HAP1-Ki67 KO. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3610 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 647 Anti-Ki67 antibody [EPR3610] (ab196907)
- Anti-Ki67 antibody [EPR3610] - BSA and Azide free (ab209897)
- Alexa Fluor® 568 Anti-Ki67 antibody [EPR3610] (ab211968)
- HRP Anti-Ki67 antibody [EPR3610] (ab212215)
- Alexa Fluor® 555 Anti-Ki67 antibody [EPR3610] (ab215226)
- Alexa Fluor® 594 Anti-Ki67 antibody [EPR3610] (ab216709)
- Anti-Ki67 antibody [EPR3610] (ab92742)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
|
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| ICC | (1) |
1/0.5 - 1/1.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 - 20 min) and 100% methanol (5 min). If fixing cells in 4% PFA, it is recommended to permeabilized cells with 0.1% Triton-X for 5 min. |
| Notes |
|---|
|
Flow Cyt (Intra)
1/50. |
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ICC
1/0.5 - 1/1. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 - 20 min) and 100% methanol (5 min). If fixing cells in 4% PFA, it is recommended to permeabilized cells with 0.1% Triton-X for 5 min. |
Target
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Function
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed. -
Sequence similarities
Contains 1 FHA domain.
Contains 16 K167R repeats.
Contains 1 PP1-binding domain. -
Developmental stage
Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815). -
Post-translational
modificationsPhosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. -
Cellular localization
Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106). - Information by UniProt
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Database links
- Entrez Gene: 4288 Human
- Omim: 176741 Human
- SwissProt: P46013 Human
- Unigene: 689823 Human
- Unigene: 80976 Human
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Alternative names
- Antigen identified by monoclonal antibody Ki 67 antibody
- Antigen identified by monoclonal antibody Ki-67 antibody
- Antigen KI-67 antibody
see all
Images
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Immunocytochemistry - Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234)
ab197234 staining Ki67 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197234 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).
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![Immunocytochemistry - Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234)](https://www.abcam.com/ps/products/197/ab197234/Images/ab197234-265350-anti-ki67-antibody-epr3610-alexa-fluor-488-immunofluorescence.jpg)
Immunocytochemistry - Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234)ab197234 staining Ki67 in wild-type HAP1 cells (top panel) and Ki67 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab197234 at 1/100 dilution (shown in green) and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234)](https://www.abcam.com/ps/products/197/ab197234/Images/ab197234-257066-anti-ki67-antibody-epr3610-alexa-fluor-488-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234)Overlay histogram showing HeLa cells stained with ab197234 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197234, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234)](https://www.abcam.com/ps/products/197/ab197234/Images/ab197234-4-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-Ki67 antibody [EPR3610] (ab197234)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (25)
ab197234 has been referenced in 25 publications.
- Guo HY et al. Effects of Bunao-Fuyuan decoction serum on proliferation and migration of vascular smooth muscle cells in atherosclerotic. Chin J Nat Med 19:36-45 (2021). PubMed: 33516450
- Meng X et al. Umbilical cord-derived mesenchymal stem cells exert anti-fibrotic action on hypertrophic scar-derived fibroblasts in co-culture by inhibiting the activation of the TGF ß1/Smad3 pathway. Exp Ther Med 21:210 (2021). PubMed: 33574910
- Guan X et al. EZH2-Mediated microRNA-375 Upregulation Promotes Progression of Breast Cancer via the Inhibition of FOXO1 and the p53 Signaling Pathway. Front Genet 12:633756 (2021). PubMed: 33854524
- Hu L et al. The anti-angiogenesis role of FBXW7 in diabetic retinopathy by facilitating the ubiquitination degradation of c-Myc to orchestrate the HDAC2. J Cell Mol Med 25:2190-2202 (2021). PubMed: 33369138
- Fang X et al. Germacrone Regulates HBXIP-Mediated Cell Cycle, Apoptosis and Promotes the Formation of Autophagosomes to Inhibit the Proliferation of Gastric Cancer Cells. Front Oncol 10:537322 (2020). PubMed: 33244453