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  1. Link

    alexa-fluor-488-lamin-a--lamin-b1--lamin-c-antibody-epr4068-ab223942.pdf

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Cell Biology Apoptosis Nucleus Lamins
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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)

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  • Certificate of Compliance
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Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)
  • Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR4068] to Lamin A + Lamin B1 + Lamin C
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free Unconjugated

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Overview

  • Product name

    Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR4068] to Lamin A + Lamin B1 + Lamin C
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Specificity

    The antibody recognizes full length Lamin A/B1/C and the cleaved small unit.

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4068
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Lamins
    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Scaffold Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Envelope
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nucleoplasm
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class V
    • Lamins
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Lamins

Associated products

  • Alternative Versions

    • Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922)
    • Alexa Fluor® 647 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223943)
    • Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - BSA and Azide free (ab226043)
  • Isotype control

    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab223942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1000.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)

Flow Cyt (Intra)
1/500.
Notes
ICC/IF
1/1000.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)

Flow Cyt (Intra)
1/500.

Target

  • Cellular localization

    Lamin A: Nucleus. Nucleus envelope. Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localization of non-farnesylated prelamin-A/C. Lamin B1: Nucleus inner membrane. Lamin C: Nucleus. Nucleus envelope. Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localization of non-farnesylated prelamin-A/C.
  • Database links

    • Entrez Gene: 4000 Human
    • Entrez Gene: 4001 Human
    • Entrez Gene: 16905 Mouse
    • Entrez Gene: 16906 Mouse
    • Entrez Gene: 116685 Rat
    • Entrez Gene: 60374 Rat
    • Omim: 150330 Human
    • Omim: 150340 Human
    • SwissProt: P02545 Human
    • SwissProt: P20700 Human
    • SwissProt: P14733 Mouse
    • SwissProt: P48678 Mouse
    • SwissProt: P48679 Rat
    • SwissProt: P70615 Rat
    • Unigene: 594444 Human
    • Unigene: 89497 Human
    • Unigene: 243014 Mouse
    • Unigene: 4105 Mouse
    • Unigene: 471227 Mouse
    • Unigene: 11362 Rat
    • Unigene: 44161 Rat
    see all
  • Form

    Lamin C: There are 3 isoforms produced by alternative splicing. Isoform A also known as Lamin A; Isoform C also known as Lamin C; Isoform ADelta10 also known as Lamin ADelta10.

Images

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)
    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)

    Overlay histogram showing HeLa cells stained with ab223942 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223942, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)

    ab223942 staining Lamin A + B1 + C in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223942 at 1/1000 dilution (shown in Green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)
    Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab223942? Please let us know so that we can cite the reference in this datasheet.

ab223942 has not yet been referenced specifically in any publications.

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