Recombinant Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)](https://www.abcam.com/ps/products/223/ab223942/Images/ab223942-318227-anti-lamin-a-c-antibody-epr4068-alexa-fluor-488-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR4068] to Lamin A + Lamin B1 + Lamin C
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
-
Product name
Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR4068] to Lamin A + Lamin B1 + Lamin C -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Specificity
The antibody recognizes full length Lamin A/B1/C and the cleaved small unit.
-
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4068 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/1000.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) |
|
| Flow Cyt (Intra) |
1/500.
|
| Notes |
|---|
|
ICC/IF
1/1000. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) |
|
Flow Cyt (Intra)
1/500. |
Target
-
Cellular localization
Lamin A: Nucleus. Nucleus envelope. Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localization of non-farnesylated prelamin-A/C. Lamin B1: Nucleus inner membrane. Lamin C: Nucleus. Nucleus envelope. Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localization of non-farnesylated prelamin-A/C. -
Database links
- Entrez Gene: 4000 Human
- Entrez Gene: 4001 Human
- Entrez Gene: 16905 Mouse
- Entrez Gene: 16906 Mouse
- Entrez Gene: 116685 Rat
- Entrez Gene: 60374 Rat
- Omim: 150330 Human
- Omim: 150340 Human
see all -
Form
Lamin C: There are 3 isoforms produced by alternative splicing. Isoform A also known as Lamin A; Isoform C also known as Lamin C; Isoform ADelta10 also known as Lamin ADelta10.
Images
-
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)
Overlay histogram showing HeLa cells stained with ab223942 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223942, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
-
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)](https://www.abcam.com/ps/products/223/ab223942/Images/ab223942-286670-anti-lamin-a-c-antibody-epr4068-alexa-fluor-488-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)ab223942 staining Lamin A + B1 + C in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223942 at 1/1000 dilution (shown in Green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
-
![Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)](https://www.abcam.com/ps/products/223/ab223942/Images/ab223942-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab223942 has not yet been referenced specifically in any publications.
