RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)

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Flow Cytometry - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)
  • Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR4068] to Lamin A + Lamin B1 + Lamin C
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068]

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR4068] to Lamin A + Lamin B1 + Lamin C

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Specificity

    The antibody recognizes full length Lamin A/B1/C and the cleaved small unit.

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Lamin A aa 1-100. The exact sequence is proprietary.
    Database link: P02545

  • Positive control

    • ICC/IF: HeLa cells. Flow Cyt: HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab223942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)
Flow Cyt 1/500.

Target

Images

  • Flow Cytometry - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)
    Flow Cytometry - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)

    Overlay histogram showing HeLa cells stained with ab223942 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223942, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)

    ab223942 staining Lamin A + B1 + C in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223942 at 1/1000 dilution (shown in Green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)
    Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab223942? Please let us know so that we can cite the reference in this datasheet.

ab223942 has not yet been referenced specifically in any publications.

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