RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-MSK1 (phospho S376) antibody [E375] (ab201759)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MSK1 (phospho S376) antibody [E375] (ab201759)
  • Alexa Fluor® 488 Anti-MSK1 (phospho S376) antibody [E375] (ab201759)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [E375] to MSK1 (phospho S376)
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-MSK1 (phospho S376) antibody [E375]
    See all MSK1 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [E375] to MSK1 (phospho S376)

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MSK1 (phospho S376).

  • Positive control

    • ICC/IF: HepG2 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab201759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function

    Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).

  • Tissue specificity

    Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.

  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.

  • Post-translational
    modifications

    Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.

  • Cellular localization

    Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.
  • Information by UniProt

  • Database links

    • Alternative names

      • 90 kDa ribosomal protein S6 kinase 5 antibody
      • EC 2.7.11.1 antibody
      • KS6A5_HUMAN antibody
      • MGC1911 antibody
      • Mitogen and stress activated protein kinase 1 antibody
      • MSPK1 antibody
      • Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody
      • Nuclear mitogen- and stress-activated protein kinase 1 antibody
      • Ribosomal protein S6 kinase 90kD polypeptide 5 antibody
      • Ribosomal protein S6 kinase 90kDa antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody
      • Ribosomal Protein S6 Kinase Alpha 5 antibody
      • Ribosomal protein S6 kinase alpha-5 antibody
      • RLPK antibody
      • RPS6KA5 antibody
      • RSK Like Protein Kinase antibody
      • RSK-like protein kinase antibody
      • RSKL antibody
      • S6K alpha 5 antibody
      • S6K-alpha-5 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MSK1 (phospho S376) antibody [E375] (ab201759)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MSK1 (phospho S376) antibody [E375] (ab201759)

      ab201759 staining MSK1 (phospho S376) in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201759 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5min).

    • Alexa Fluor® 488 Anti-MSK1 (phospho S376) antibody [E375] (ab201759)
      Alexa Fluor® 488 Anti-MSK1 (phospho S376) antibody [E375] (ab201759)

    References (0)

    Publishing research using ab201759? Please let us know so that we can cite the reference in this datasheet.

    ab201759 has not yet been referenced specifically in any publications.

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