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Signal Transduction Metabolism Amino Acids
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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-PAH antibody [EPR12380] (ab208418)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PAH antibody [EPR12380] (ab208418)
  • Alexa Fluor® 488 Anti-PAH antibody [EPR12380] (ab208418)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR12380] to PAH
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

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Overview

  • Product name

    Alexa Fluor® 488 Anti-PAH antibody [EPR12380]
    See all PAH primary antibodies
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR12380] to PAH
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HepG2 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12380
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Alternative Versions

    • Anti-PAH antibody [EPR12380] (ab178430)
    • Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)
    • Alexa Fluor® 647 Anti-PAH antibody [EPR12380] (ab207388)
  • Isotype control

    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)
  • Recombinant Protein

    • Recombinant Human PAH protein (ab159048)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab208418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
All applications
Mouse
Rat
Application Abreviews Notes
ICC/IF
1/50.

This product gave a positive signal in HepG2 cells fixed with 100% methanol (5 min)

Notes
ICC/IF
1/50.

This product gave a positive signal in HepG2 cells fixed with 100% methanol (5 min)

Target

  • Pathway

    Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 1/6.
  • Involvement in disease

    Defects in PAH are the cause of phenylketonuria (PKU) [MIM:261600]. PKU is an autosomal recessive inborn error of phenylalanine metabolism, due to severe phenylalanine hydroxylase deficiency. It is characterized by blood concentrations of phenylalanine persistently above 1200 mumol (normal concentration 100 mumol) which usually causes mental retardation (unless low phenylalanine diet is introduced early in life). They tend to have light pigmentation, rashes similar to eczema, epilepsy, extreme hyperactivity, psychotic states and an unpleasant 'mousy' odor.
    Defects in PAH are the cause of non-phenylketonuria hyperphenylalaninemia (Non-PKU HPA) [MIM:261600]. Non-PKU HPA is a mild form of phenylalanine hydroxylase deficiency characterized by phenylalanine levels persistently below 600 mumol, which allows normal intellectual and behavioral development without treatment. Non-PKU HPA is usually caused by the combined effect of a mild hyperphenylalaninemia mutation and a severe one.
    Defects in PAH are the cause of hyperphenylalaninemia (HPA) [MIM:261600]. HPA is the mildest form of phenylalanine hydroxylase deficiency.
  • Sequence similarities

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
    Contains 1 ACT domain.
  • Target information above from: UniProt accession P00439 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5053 Human
    • Entrez Gene: 18478 Mouse
    • Entrez Gene: 24616 Rat
    • Omim: 612349 Human
    • SwissProt: P00439 Human
    • SwissProt: P16331 Mouse
    • SwissProt: P04176 Rat
    • Unigene: 603740 Human
    • Unigene: 263539 Mouse
    • Unigene: 1652 Rat
    see all
  • Alternative names

    • PAH antibody
    • PH antibody
    • PH4H_HUMAN antibody
    • Phe 4 monooxygenase antibody
    • Phe-4-monooxygenase antibody
    • Phenylalanine 4 hydroxylase antibody
    • Phenylalanine hydroxylase antibody
    • Phenylalanine-4-hydroxylase antibody
    • PKU antibody
    • PKU1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PAH antibody [EPR12380] (ab208418)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PAH antibody [EPR12380] (ab208418)

    ab208418 staining PAH in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208418 at a 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Please note nucleoli fixation artefact.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-PAH antibody [EPR12380] (ab208418)
    Alexa Fluor® 488 Anti-PAH antibody [EPR12380] (ab208418)

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab208418? Please let us know so that we can cite the reference in this datasheet.

ab208418 has not yet been referenced specifically in any publications.

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