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    alexa-fluor-488-pd-l1-antibody-28-8-ab209959.pdf

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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (2)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)
  • Flow Cytometry - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)
  • Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [28-8] to PD-L1
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free FITC HRP PE Unconjugated

You may also be interested in

Isotype
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Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
Primary
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Anti-PD-L1 antibody [28-8] - Low endotoxin, Azide free (ab209889)
Primary
Product image
Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain (ab209960)

View more associated products

Overview

  • Product name

    Alexa Fluor® 488 Anti-PD-L1 antibody [28-8]
    See all PD-L1 primary antibodies
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [28-8] to PD-L1
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human PD-L1 (extracellular). The immunogen contains the specific extracellular domain of huPD-L1 (Phe19-Thr239). See reference for more info - www.ncbi.nlm.nih.gov/pmc/articles/PMC4561627/
    Database link: Q9NZQ7

  • Positive control

    • ICC/IF: PDL1 transfected CHO cells. Flow Cyt: PDL1 transfected CHO cells.
  • General notes

    Anti-PD-L1 antibody [28-8] has been used as detector antibody in Human PD-L1 SimpleStep ELISA® kit (ab214565).

    Additional information on positive controls:

    Cell Lines:
    Positives: B-CPAP- high, ES-2- medium, HCC70 - low

    For primary negative control, isotype control, RabMAb negative control antibody (ab172730) is recommended.

    For negative control sample, cell line COLO205 is recommended.

    For PD-L1 protein, see ab167713.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    28-8
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Cancer
    • Tumor biomarkers
    • Tumor antigens

Associated products

  • Alternative Versions

    • Anti-PD-L1 antibody [28-8] (ab205921)
    • APC Anti-PD-L1 antibody [28-8] (ab206967)
    • Anti-PD-L1 antibody [28-8] - Low endotoxin, Azide free (ab209889)
    • Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain (ab209960)
    • HRP Anti-PD-L1 antibody [28-8] (ab209961)
    • PE Anti-PD-L1 antibody [28-8] (ab209962)
    • Alexa Fluor® 555 Anti-PD-L1 antibody [28-8] (ab213358)
    • Alexa Fluor® 568 Anti-PD-L1 antibody [28-8] (ab213359)
    • Alexa Fluor® 594 Anti-PD-L1 antibody [28-8] (ab213360)
    • FITC Anti-PD-L1 antibody [28-8] (ab224027)
    • Anti-PD-L1 antibody [28-8] - BSA and Azide free (ab228413)
  • Isotype control

    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
  • Recombinant Protein

    • Recombinant human PD-L1 protein (Active) (ab167713)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab209959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (1)
1/50.
ICC/IF
1/100.

This product gave a positive signal in CHO-PDL1 cells fixed with 4% formaldehyde (10 min)

Notes
Flow Cyt
1/50.
ICC/IF
1/100.

This product gave a positive signal in CHO-PDL1 cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
  • Tissue specificity

    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane and Endomembrane system.
  • Target information above from: UniProt accession Q9NZQ7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 29126 Human
    • Omim: 605402 Human
    • SwissProt: Q9NZQ7 Human
    • Unigene: 521989 Human
    • Alternative names

      • B7 H antibody
      • B7 H1 antibody
      • B7 homolog 1 antibody
      • B7-H1 antibody
      • B7H antibody
      • B7H1 antibody
      • CD 274 antibody
      • CD-274 antibody
      • CD274 antibody
      • CD274 antigen antibody
      • CD274 molecule antibody
      • MGC142294 antibody
      • MGC142296 antibody
      • OTTHUMP00000021029 antibody
      • PD L1 antibody
      • PD-L1 antibody
      • PD1L1_HUMAN antibody
      • PDCD1 ligand 1 antibody
      • PDCD1L1 antibody
      • PDCD1LG1 antibody
      • PDL 1 antibody
      • PDL1 antibody
      • Programmed cell death 1 ligand 1 antibody
      • Programmed death ligand 1 antibody
      • RGD1566211 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)

      Ab209959 staining PDL1 in CHO-PDL1 cells. The lower panels demonstrate that ab209959 does not cross react with un-transfected CHO cells.

      The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209959 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)
      Flow Cytometry - Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)

      Overlay histgram showing CHO (blue line) and PDL1 transfected CHO (red line) cells stained with ab209959.

      The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209959, 1/50 dilution) for 30 min at 4ºC.

      Acquisition of >5,000 events were collected using a 20mW solid-state laser (488nm) and 530/30 bandpass filter.

    • Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)
      Alexa Fluor® 488 Anti-PD-L1 antibody [28-8] (ab209959)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (2)

    Publishing research using ab209959? Please let us know so that we can cite the reference in this datasheet.

    ab209959 has been referenced in 2 publications.

    • Chen JY  et al. Lysine demethylase 2A expression in cancer-associated fibroblasts promotes breast tumour growth. Br J Cancer 124:484-493 (2021). PubMed: 33024266
    • Yu W  et al. Autophagy inhibitor enhance ZnPc/BSA nanoparticle induced photodynamic therapy by suppressing PD-L1 expression in osteosarcoma immunotherapy. Biomaterials 192:128-139 (2019). Flow Cyt . PubMed: 30448697

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Flow Cytometry abreview for Alexa Fluor® 488 Anti-PD-L1 antibody [28-8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (AML Cell Line (MLL-AF4))
    Permeabilization
    No
    Specification
    AML Cell Line (MLL-AF4)
    Preparation
    Cell harvesting/tissue preparation method: 1 x 10^6 cells/mL in ice-cold PBS and 10% FCS
    Sample buffer: Ice-cold PBS and 10% FCS
    Fixation
    None
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 23 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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