RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] (ab202671)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] (ab202671)
  • Alexa Fluor® 488 Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] (ab202671)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EP383Y] to PI 3 Kinase catalytic subunit alpha/PIK3CA
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y]
    See all PI 3 Kinase catalytic subunit alpha/PIK3CA primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EP383Y] to PI 3 Kinase catalytic subunit alpha/PIK3CA

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human PI 3 Kinase catalytic subunit alpha/PIK3CA aa 1000-1100 (C terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab202671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

Target

  • Function

    Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.

  • Involvement in disease

    Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500].
    Defects in PIK3CA are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
    Defects in PIK3CA are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Defects in PIK3CA may underlie hepatocellular carcinoma (HCC) [MIM:114550].
    Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB) [MIM:182000]. A common benign skin tumor. Seborrheic keratoses usually begin with the appearance of one or more sharply defined, light brown, flat macules. The lesions may be sparse or numerous. As they initially grow, they develop a velvety to finely verrucous surface, followed by an uneven warty surface with multiple plugged follicles and a dull or lackluster appearance.

  • Sequence similarities

    Belongs to the PI3/PI4-kinase family.
    Contains 1 C2 domain.
    Contains 1 PI3K/PI4K domain.
  • Information by UniProt

  • Database links

    • Alternative names

      • 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody
      • 5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody
      • caPI3K antibody
      • CLOVE antibody
      • CWS5 antibody
      • MCAP antibody
      • MCM antibody
      • MCMTC antibody
      • MGC142161 antibody
      • MGC142163 antibody
      • p110 alpha antibody
      • p110alpha antibody
      • Phosphatidylinositol 3 kinase catalytic alpha polypeptide antibody
      • Phosphatidylinositol 3 kinase catalytic 110 KD alpha antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform antibody
      • Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha antibody
      • Phosphatidylinositol-4 antibody
      • Phosphoinositide 3 kinase catalytic alpha polypeptide antibody
      • PI 3 Kinase catalytic subunit alpha antibody
      • PI3 kinase p110 subunit alpha antibody
      • PI3-kinase subunit alpha antibody
      • PI3K antibody
      • PI3K-alpha antibody
      • PI3KC A antibody
      • PIK3C A antibody
      • Pik3ca antibody
      • PK3CA antibody
      • PK3CA_HUMAN antibody
      • PtdIns 3 kinase p110 antibody
      • PtdIns-3-kinase subunit alpha antibody
      • PtdIns-3-kinase subunit p110-alpha antibody
      • Serine/threonine protein kinase PIK3CA antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] (ab202671)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] (ab202671)

      ab202671 staining PI 3 Kinase catalytic subunit alpha/PIK3CA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202671 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

    • Alexa Fluor® 488 Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] (ab202671)
      Alexa Fluor® 488 Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] (ab202671)

    References (0)

    Publishing research using ab202671? Please let us know so that we can cite the reference in this datasheet.

    ab202671 has not yet been referenced specifically in any publications.

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