RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab225297)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab225297)
  • Alexa Fluor® 488 Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab225297)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EP12357] to Prostaglandin D Synthase (Lipocalin)/PDS
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357]
    See all Prostaglandin D Synthase (Lipocalin)/PDS primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EP12357] to Prostaglandin D Synthase (Lipocalin)/PDS

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Prostaglandin D Synthase (Lipocalin)/PDS aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P41222
    (Peptide available as ab188544)

  • Positive control

    • ICC/IF: HepG2 cells.

  • General notes

     This product was previously labelled as Prostaglandin D Synthase (Lipocalin)

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP12357

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.

This product gave a positive signal in HepG2 cells fixed with 100% methanol (5 min)

Target

  • Function

    Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.

  • Tissue specificity

    Abundant in the brain and CNS, where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. Abundantly expressed in the heart. In the male reproductive system, it is expressed in the testis, epididymis and prostate, and is secreted into the seminal fluid. Expressed in the eye and secreted into the aqueous humor. Lower levels detected in various tissue fluids such as serum, normal urine, ascitic fluid and tear fluid. Also found in a number of other organs including ovary, fimbriae of the fallopian tubes, kidney, leukocytes.

  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.

  • Developmental stage

    Expression in the amniotic fluid increases dramatically during weeks 12 to 25 of pregnancy. Levels decrease slowly after 25 weeks.

  • Domain

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.

  • Post-translational
    modifications

    Both N-glycosylation recognition sites are almost quantitatively occupied by N-glycans of the biantennary complex type, with a considerable proportion of structures bearing a bisecting GlcNAc. Agalacto structure as well as sialylated and nonsialylated oligosaccharides bearing alpha2-3- and/or alpha2-6-linked NeuNAc are present.

  • Cellular localization

    Rough endoplasmic reticulum. Nucleus membrane. Golgi apparatus. Cytoplasm > perinuclear region. Secreted. Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Beta trace protein antibody
    • Beta-trace protein antibody
    • Cerebrin 28 antibody
    • Cerebrin-28 antibody
    • glutathione independent PGD synthase antibody
    • Glutathione independent PGD synthetase antibody
    • Glutathione-independent PGD synthase antibody
    • L PGDS antibody
    • Lipocalin type prostaglandin D synthase antibody
    • Lipocalin-type prostaglandin-D synthase antibody
    • LPGDS antibody
    • PDS antibody
    • PGD2 antibody
    • PGD2 synthase antibody
    • PGDS antibody
    • PGDS2 antibody
    • prostaglandin D synthase antibody
    • prostaglandin D2 synthase (21 kDa, brain) antibody
    • prostaglandin D2 synthase (brain) antibody
    • prostaglandin D2 synthase 21kDa (brain) antibody
    • Prostaglandin D2 synthase antibody
    • prostaglandin H2 D isomerase antibody
    • Prostaglandin-D2 synthase antibody
    • Prostaglandin-H2 D-isomerase antibody
    • PTGDS antibody
    • PTGDS_HUMAN antibody
    • RP23-47P18.11-006 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab225297)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab225297)

    ab225297 staining Prostaglandin D Synthase (Lipocalin)/PDS in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225297 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab225297)
    Alexa Fluor® 488 Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab225297)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab225297? Please let us know so that we can cite the reference in this datasheet.

ab225297 has not yet been referenced specifically in any publications.

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