Key features and details
- Alexa Fluor® 488 Mouse monoclonal [2E3GC12FB2AE2] to SDHA
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Mouse, Rat, Cow, Dog, Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Product nameAlexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2]
See all SDHA primary antibodies
DescriptionAlexa Fluor® 488 Mouse monoclonal [2E3GC12FB2AE2] to SDHA
ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
Tested applicationsSuitable for: ICC/IF, Flow Cyt, WBmore details
Species reactivityReacts with: Mouse, Rat, Cow, Dog, Human
Full length native protein (purified) corresponding to Cow SDHA.
- HeLa and HDFn cells.
Product was previously marketed under the MitoSciences sub-brand.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS, 30% Glycerol
Concentration information loading...
Purification notesPurity is near homogeneity as judged by SDS-PAGE. ab154473 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab154473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use a concentration of 0.2 µg/ml.
ab171463 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionFlavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
PathwayCarbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
Involvement in diseaseDefects in SDHA are a cause of mitochondrial complex II deficiency (MT-C2D) [MIM:252011]. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations. Clinical features include psychomotor regression in infants, poor growth with lack of speech development, severe spastic quadriplegia, dystonia, progressive leukoencephalopathy, muscle weakness, exercise intolerance, cardiomyopathy. Some patients manifest Leigh syndrome or Kearns-Sayre syndrome.
Defects in SDHA are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
Defects in SDHA are the cause of cardiomyopathy dilated type 1GG (CMD1GG) [MIM:613642]. CMD1GG is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesBelongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- CMD1GG antibody
- DHSA_HUMAN antibody
- Flavoprotein subunit of complex II antibody
ab154473 staining SDHA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab154473 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Clone 2E3GC12FB2AE2 has been knock-out validated in its unconjugated form. Please refer to ab14715 datasheet for experimental details.
Immunocytochemistry with HeLa cells (100x) were stained with anti-SDHA Alexa-488 antibody (1.0 µg/mL, ab154473) in green and DAPI in blue, as a nuclear stain.
Flow Cytometry for Anti-SDHA Alexa-488 antibody (ab154473) stained HeLa cells. Flow cytometry results of unstained cells (black) and 0.2 µg/mL anti-SDHA Alexa-488 (red) stained cells.
ab154473 has not yet been referenced specifically in any publications.