RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-SOX2 antibody [EPR3131] (ab195358)

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Flow Cytometry - Alexa Fluor® 488 Anti-SOX2 antibody [EPR3131] (ab195358)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SOX2 antibody [EPR3131] (ab195358)
  • Alexa Fluor® 488 Anti-SOX2 antibody [EPR3131] (ab195358)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR3131] to SOX2
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-SOX2 antibody [EPR3131]
    See all SOX2 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR3131] to SOX2

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: MCF7 cells. Flow Cyt: MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab195358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
Application Abreviews Notes
Flow Cyt
1/500.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.
ICC/IF
1/100.
Notes
Flow Cyt
1/500.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.
ICC/IF
1/100.

Target

  • Function

    Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.

  • Involvement in disease

    Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.

  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.

  • Post-translational
    modifications

    Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • ANOP3 antibody
      • cb236 antibody
      • Delta EF2a antibody
      • lcc antibody
      • MCOPS3 antibody
      • MGC148683 antibody
      • MGC2413 antibody
      • RGD1565646 antibody
      • Sex determining region Y box 2 antibody
      • SOX 2 antibody
      • Sox2 antibody
      • SOX2_HUMAN antibody
      • SRY (sex determining region Y) box 2 antibody
      • SRY box containing gene 2 antibody
      • SRY related HMG box 2 antibody
      • SRY related HMG box gene 2 antibody
      • SRY-box 2 antibody
      • Transcription factor SOX 2 antibody
      • Transcription factor SOX-2 antibody
      • ysb antibody
      see all

    Images

    • Flow Cytometry - Alexa Fluor® 488 Anti-SOX2 antibody [EPR3131] (ab195358)
      Flow Cytometry - Alexa Fluor® 488 Anti-SOX2 antibody [EPR3131] (ab195358)

      Overlay histogram showing MCF7 cells stained with ab195358 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195358, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SOX2 antibody [EPR3131] (ab195358)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SOX2 antibody [EPR3131] (ab195358)

      ab195358 staining SOX2 in MCF-7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195358 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 488 Anti-SOX2 antibody [EPR3131] (ab195358)
      Alexa Fluor® 488 Anti-SOX2 antibody [EPR3131] (ab195358)

    References (0)

    Publishing research using ab195358? Please let us know so that we can cite the reference in this datasheet.

    ab195358 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Immunohistochemistry (Frozen sections) abreview for Alexa Fluor® 488 Anti-SOX2 antibody [EPR3131]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rabbit Tissue sections (Brain (Coronal section))
    Permeabilization
    No
    Specification
    Brain (Coronal section)
    Blocking step
    NDS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Govindaiah Vinukonda

    Verified customer

    Submitted Jan 08 2021

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