Recombinant Alexa Fluor® 488 Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab201858)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EP503Y] to SRC Family (phospho Y418)
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Overview
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Product name
Alexa Fluor® 488 Anti-SRC Family (phospho Y418) antibody [EP503Y]
See all SRC Family primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EP503Y] to SRC Family (phospho Y418) -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human SRC Family aa 350-450 (phospho Y418). The exact sequence is proprietary.
Database link: P07948 -
Positive control
- ICC/IF: HeLa cells +/- pervanadate (Non-treated: serum-starved for 16h at 37°C; Treated: serum-starved for 16h, then incubated with pervanadate 1mM for 30min at 37°C).
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General notes
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP503Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 647 Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab201860)
- Alexa Fluor® 594 Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab206347)
- PE Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab208828)
- Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (ab239824)
- Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab40660)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab201858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | 1/100. |
Target
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Relevance
SRC Family Negative Regulatory Phosphorylation Site belongs to the protein kinase superfamily, Tyr protein kinase family, SRC subfamily. It contains one protein kinase domain, one SH2 domain and one SH3 domain. It may serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst and may also contribute to neutrophil migration and may regulate the degranulation process of neutrophils. SRC Family Negative Regulatory Phosphorylation Site is expressed predominantly in cells of the myeloid and B lymphoid lineages. There are two named isoforms. -
Cellular localization
Isoform 1: Membrane; Lipid anchor. Isoform 2: Membrane; Lipid anchor. Cytoplasm. -
Database links
- Entrez Gene: 6714 Human
- Entrez Gene: 20779 Mouse
- Omim: 190090 Human
- SwissProt: P12931 Human
- SwissProt: P05480 Mouse
- Unigene: 195659 Human
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Alternative names
- ASV antibody
- Protooncogene SRC, Rous sarcoma antibody
- SRC antibody
- SRC1 antibody
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab201858)
ab201858 staining SRC Family (phospho Y418) in HeLa cells +/- pervanadate (1mM, 15min), then treated with LP (31 degrees C, 2 hours). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.
The cells were then incubated overnight at +4°C with ab201858 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab201858)
ab201858 staining Lyn (phospho Y396) in HeLa cells +/- pervanadate (Non-treated: serum-starved for 16h at 37°C; Treated: serum-starved for 16h, then incubated with pervanadate 1mM for 30min at 37°C). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201858 at 1/250 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594) at 1/200 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab201858 has not yet been referenced specifically in any publications.