RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab216934)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab216934)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab216934)
  • Alexa Fluor® 488 Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab216934)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR15556] to Tartrate Resistant Acid Phosphatase
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556]
    See all Tartrate Resistant Acid Phosphatase primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR15556] to Tartrate Resistant Acid Phosphatase

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human Tartrate Resistant Acid Phosphatase aa 150-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P13686

  • Positive control

    • ICC/IF: MDA-MB-435 and HACAT cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab216934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
All applications
Mouse
Rat
Application Abreviews Notes
ICC/IF
1/50.

This product gave a positive signal in MDA-MB-435 and HACAT cells fixed with 100% methanol (5 min)
Notes
ICC/IF
1/50.

This product gave a positive signal in MDA-MB-435 and HACAT cells fixed with 100% methanol (5 min)

Target

  • Function

    Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.

  • Sequence similarities

    Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

  • Cellular localization

    Lysosome.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Type 5 acid phosphatase antibody
    • Acid phosphatase 5 tartrate resistant antibody
    • Acid phosphatase 5, tartrate resistant antibody
    • ACP5 antibody
    • EC 3.1.3.2 antibody
    • MGC117378 antibody
    • PPA5_HUMAN antibody
    • serum band 5 tartrate-resistant acid phosphatase antibody
    • SPENCDI antibody
    • T5ap antibody
    • Tartrate resistant acid ATPase antibody
    • Tartrate resistant acid phosphatase type 5 antibody
    • Tartrate resistant acid phosphatase type 5 precursor antibody
    • Tartrate-resistant acid ATPase antibody
    • Tartrate-resistant acid phosphatase type 5 antibody
    • TR AP antibody
    • TR-AP antibody
    • TRACP 5 antibody
    • TRAP antibody
    • TrATPase antibody
    • Type 5 acid phosphatase antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab216934)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab216934)

    ab216934 staining Tartrate Resistant Acid Phosphatase in HACAT cells. The cells were fixed with 100% methanol (5 min), and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions.

    The cells were then incubated overnight at +4°C with ab216934 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

     

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab216934)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab216934)

    ab216934 staining Tartrate Resistant Acid Phosphatase in MDA-MB-435 cells. The cells were fixed with 100% methanol (5 min), and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions.

    The cells were then incubated overnight at +4°C with ab216934 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab216934)
    Alexa Fluor® 488 Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab216934)

References (2)

Publishing research using ab216934? Please let us know so that we can cite the reference in this datasheet.

ab216934 has been referenced in 2 publications.

  • Igari K  et al. Cigarette Smoke Extract Activates Tartrate-Resistant Acid Phosphatase-Positive Macrophage. J Vasc Res 56:139-151 (2019). PubMed: 31064000
  • Igari K  et al. Digoxin Attenuates Receptor Activation of NF-?B Ligand-Induced Osteoclastogenesis in Macrophages. J Vasc Res 56:55-64 (2019). PubMed: 31085912

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