Validated using a knockout cell lineRecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)

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Flow Cytometry - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)
  • Flow Cytometry - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)
  • Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [106-6] to TMEM119
  • Suitable for: Flow Cyt
  • Knockout validated
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-TMEM119 antibody [106-6]
    See all TMEM119 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [106-6] to TMEM119

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment (MBP-tag) within Mouse TMEM119 aa 1-100 (extracellular). The exact sequence is proprietary.
    Database link: Q8R138

  • Positive control

    • Flow Cyt: HEK-293T transfected with Myc-His tagged TMEM119 expression vector; CD11b+ brain cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Monoclonal

  • Clone number

    106-6

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

The cellular localisation of this product has been verified in ICC/IF.

This antibody may be used at 10 μg/mL.

Target

  • Cellular localization

    Membrane; Single-pass type I membrane protein

  • Database links

    • Alternative names

      • OBIF antibody
      • Osteoblast induction factor antibody
      • PSEC0199 antibody
      • Transmembrane protein 119 antibody
      • UNQ731/PRO1415 antibody
      see all

    Images

    • Flow Cytometry - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)
      Flow Cytometry - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)

      Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225497 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Alexa Fluor® 488 ab199091 (left). Cells were surface-stained with ab225497, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

    • Flow Cytometry - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)
      Flow Cytometry - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)This image is courtesy of an anonymous collaborator.

      Flow cytometric analysis of wild type (WT) versus Tmem119 knockout (KO) CD11b+ cells labeling TMEM119 with ab225497 at 10 μg/mL (green) or the same clone unconjugated (ab210405) at 1 μg/mL with an Alexa 488 secondary antibody (purple), compared with TMEM119 KO primary mouse brain cells stained with ab210405 at 1 μg/mL (black) and ab225497 at 10 μg/mL (grey). Goat anti-Rabbit IgG (Alexa Fluor®488) at 1:1000 dilution was used as the secondary antibody.

      No signal was detected on the surface of CD11b+ brain cells from TMEM119 KO mouse stained with either the unconjugated ab210405 (black) or conjugated ab225497 antibodies (grey); whereas in wildtype CD11b+ brain cells, cell surface staining was observed (green, Alexa conjugated ab225497; purple, unconjugated ab210405 plus secondary antibody).

    • Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)
      Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] (ab225497)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab225497? Please let us know so that we can cite the reference in this datasheet.

    ab225497 has not yet been referenced specifically in any publications.

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