RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-Topoisomerase I antibody [EPR5376(2)] (ab223421)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Topoisomerase I antibody [EPR5376(2)] (ab223421)
  • Alexa Fluor® 488 Anti-Topoisomerase I antibody [EPR5376(2)] (ab223421)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR5376(2)] to Topoisomerase I
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Overview

  • Product name

    Alexa Fluor® 488 Anti-Topoisomerase I antibody [EPR5376(2)]
    See all Topoisomerase I primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR5376(2)] to Topoisomerase I

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: MCF7 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5376(2)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab223421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
All applications
Mouse
Rat
Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in MCF7 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)
Notes
ICC/IF
1/100.

This product gave a positive signal in MCF7 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Function

    The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.

  • Involvement in disease

    Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.

  • Sequence similarities

    Belongs to the eukaryotic type I topoisomerase family.

  • Post-translational
    modifications

    Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.

  • Cellular localization

    Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DNA topoisomerase 1 antibody
    • DNA topoisomerase I antibody
    • NUP98 fusion gene antibody
    • TOP 1 antibody
    • TOP I antibody
    • TOP1 antibody
    • TOP1_HUMAN antibody
    • TOPI antibody
    • Topoisomerase (DNA) I antibody
    • Topoisomerase 1 antibody
    • Topoisomerase1 antibody
    • TopoisomeraseI antibody
    • Type I DNA topoisomerase antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Topoisomerase I antibody [EPR5376(2)] (ab223421)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Topoisomerase I antibody [EPR5376(2)] (ab223421)

    ab223421 staining Topoisomerase I in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223421 at 1/100 dilution (shown in Green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 100% methanol (5 min).

  • Alexa Fluor® 488 Anti-Topoisomerase I antibody [EPR5376(2)] (ab223421)
    Alexa Fluor® 488 Anti-Topoisomerase I antibody [EPR5376(2)] (ab223421)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab223421? Please let us know so that we can cite the reference in this datasheet.

ab223421 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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