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    alexa-fluor-488-tubulin-antibody-yol134-microtubule-marker-ab195883.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Microtubules
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Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195883)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195883)

    Key features and details

    • Alexa Fluor® 488 Rat monoclonal [YOL1/34] to Tubulin - Microtubule Marker
    • Suitable for: ICC/IF
    • Reacts with: Mouse, Rat, Dog, Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Alligator
    • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
    • Isotype: IgG2a

    You may also be interested in

    Primary
    Product image
    Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884)

    View more associated products

    Overview

    • Product name

      Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker
      See all Tubulin primary antibodies
    • Description

      Alexa Fluor® 488 Rat monoclonal [YOL1/34] to Tubulin - Microtubule Marker
    • Host species

      Rat
    • Conjugation

      Alexa Fluor® 488. Ex: 495nm, Em: 519nm
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Dog, Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Alligator
    • Immunogen

      Full length native protein (purified) corresponding to Saccharomyces cerevisiae Tubulin.

    • Positive control

      • ICC/IF: HeLa cells.
    • General notes

       

       

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 30% Glycerol, 1% BSA, PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Monoclonal
    • Clone number

      YOL1/34
    • Isotype

      IgG2a
    • Research areas

      • Tags & Cell Markers
      • Subcellular Markers
      • Cytoskeleton
      • Microtubules
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microtubules
      • Tubulin
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Axon marker

    Associated products

    • Alternative Versions

      • Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884)
      • HRP Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab196583)
      • Anti-Tubulin antibody [YOL1/34] - BSA and Azide free (ab264065)
      • Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

    Applications

    Our Abpromise guarantee covers the use of ab195883 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/100.

    This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

    Target

    • Function

      Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
    • Sequence similarities

      Belongs to the tubulin family.
    • Post-translational
      modifications

      Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
      Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
      Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Target information above from: UniProt accession P68363 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10376 Human
      • Entrez Gene: 203068 Human
      • Entrez Gene: 7280 Human
      • Entrez Gene: 22143 Mouse
      • Entrez Gene: 22151 Mouse
      • Entrez Gene: 22153 Mouse
      • Entrez Gene: 500929 Rat
      • Omim: 191130 Human
      • Omim: 602530 Human
      • Omim: 615101 Human
      • SwissProt: P07437 Human
      • SwissProt: P68363 Human
      • SwissProt: Q13885 Human
      • SwissProt: P05213 Mouse
      • SwissProt: Q7TMM9 Mouse
      • SwissProt: Q9D6F9 Mouse
      • SwissProt: Q6P9V9 Rat
      • Unigene: 524390 Human
      • Unigene: 636480 Human
      • Unigene: 654543 Human
      • Unigene: 392113 Mouse
      • Unigene: 422827 Mouse
      • Unigene: 7420 Mouse
      • Unigene: 99661 Rat
      see all
    • Alternative names

      • Tubulin beta 2b antibody
      • Alpha tubulin antibody
      • Alpha-tubulin ubiquitous antibody
      • beta Ib tubulin antibody
      • CDCBM5 antibody
      • CDCBM6 antibody
      • fd02b12 antibody
      • K ALPHA 1 antibody
      • M40 antibody
      • OK/SW-cl.56 antibody
      • TBA1B_HUMAN antibody
      • TUBA1B antibody
      • TUBB1 antibody
      • TUBB2 antibody
      • TUBB5 antibody
      • tubulin alpha 1b antibody
      • Tubulin alpha-1B chain antibody
      • Tubulin alpha-ubiquitous chain antibody
      • Tubulin beta 1b antibody
      • Tubulin beta 2A antibody
      • tubulin beta 2A class IIa antibody
      • Tubulin beta antibody
      • tubulin beta chain antibody
      • tubulin beta class I antibody
      • tubulin beta-1 chain antibody
      • tubulin beta-2A chain antibody
      • tubulin beta-5 chain antibody
      • Tubulin K-alpha-1 antibody
      • tubulin, alpha, ubiquitous antibody
      • tubulin, beta 2A class IIa antibody
      • tubulin, beta polypeptide 2 antibody
      • tubulin, beta polypeptide antibody
      • tubulin, beta, class IIA antibody
      • wu:fd02b12 antibody
      • zgc:55461 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195883)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195883)

      ab195883 staining tubulin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195883 at a 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab195883? Please let us know so that we can cite the reference in this datasheet.

    ab195883 has been referenced in 2 publications.

    • Pocasap P  et al. Alyssin and Iberin in Cruciferous Vegetables Exert Anticancer Activity in HepG2 by Increasing Intracellular Reactive Oxygen Species and Tubulin Depolymerization. Biomol Ther (Seoul) 27:540-552 (2019). PubMed: 31405267
    • Pocasap P  et al. Structures of isothiocyanates attributed to reactive oxygen species generation and microtubule depolymerization in HepG2 cells. Biomed Pharmacother 101:698-709 (2018). PubMed: 29522950

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (Alexa Fluor® 488)

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Hydra vulgaris Tissue sections (cell type)
    Antigen retrieval step
    None
    Permeabilization
    Yes - triton
    Specification
    cell type
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Dr. Liora Garion

    Verified customer

    Submitted Mar 27 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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