Alexa Fluor® 555 Anti-Aurora A antibody [35C1] (ab217712)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Aurora A antibody [35C1] (ab217712)](https://www.abcam.com/ps/products/217/ab217712/Images/ab217712-275468-anti-aurora-a-antibody-35c1-alexa-fluor-555-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Aurora A antibody [35C1] (ab217712)")
Key features and details
- Alexa Fluor® 555 Mouse monoclonal [35C1] to Aurora A
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
- Isotype: IgG2b
Overview
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Product name
Alexa Fluor® 555 Anti-Aurora A antibody [35C1]
See all Aurora A primary antibodies -
Description
Alexa Fluor® 555 Mouse monoclonal [35C1] to Aurora A -
Host species
Mouse -
Conjugation
Alexa Fluor® 555. Ex: 555nm, Em: 565nm -
Specificity
The specificity of ab217712 was confirmed by showing that it binds in ELISA to recombinant Aurora A, it detects a 46kd band on HeLa cell extract and it binds to duplicated centrosomes and spindle poles in MCF7 cells. This antibody does not inhibit the kinase activity of Aurora A. This antibody has been successfully used to detect Aurora A in the following cell extracts: 293T, HMEC, T47D, MCF7, MDA-MB-468, SK-Br-3, S68 and HeLa. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant full length protein (His-tag) corresponding to Human Aurora A.
Database link: O14965 -
Positive control
- ICC/IF: HeLa cells
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
35C1 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab217712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/1000. This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min) |
Target
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Function
Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. -
Tissue specificity
Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsActivated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis. - Information by UniProt
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Database links
- Entrez Gene: 6790 Human
- Entrez Gene: 20878 Mouse
- Omim: 603072 Human
- SwissProt: O14965 Human
- SwissProt: P97477 Mouse
- Unigene: 250822 Human
- Unigene: 249363 Mouse
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Alternative names
- AIK antibody
- ARK-1 antibody
- ARK1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Aurora A antibody [35C1] (ab217712)
ab217712 staining Aurora A in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab217712 at 1/1000 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
Datasheets and documents
References (0)
ab217712 has not yet been referenced specifically in any publications.
