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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Alexa Fluor® 555 Anti-BRD2 antibody [EPR7642] (ab213347)

Submit a review Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-BRD2 antibody [EPR7642] (ab213347)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-BRD2 antibody [EPR7642] (ab213347)
  • Alexa Fluor® 555 Anti-BRD2 antibody [EPR7642] (ab213347)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [EPR7642] to BRD2
  • Suitable for: ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP Unconjugated

Overview

  • Product name

    Alexa Fluor® 555 Anti-BRD2 antibody [EPR7642]
    See all BRD2 primary antibodies

  • Description

    Alexa Fluor® 555 Rabbit monoclonal [EPR7642] to BRD2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells and wildtype HAP1 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab213347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1000 - 1/500.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)
Notes
ICC/IF
1/1000 - 1/500.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Function

    May play a role in spermatogenesis or folliculogenesis.

  • Sequence similarities

    Contains 2 bromo domains.
    Contains 1 ET domain.

  • Domain

    One bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • BRD 2 antibody
      • Brd2 antibody
      • BRD2_HUMAN antibody
      • Bromodomain containing 2 antibody
      • Bromodomain-containing protein 2 antibody
      • D6S113E antibody
      • DKFZp686N0336 antibody
      • Female sterile homeotic related gene 1 antibody
      • Female sterile homeotic related gene 1, mouse, homolog of antibody
      • FLJ31942 antibody
      • FSH antibody
      • FSRG1 antibody
      • KIAA9001 antibody
      • NAT antibody
      • O27.1.1 antibody
      • Really interesting new gene 3 protein antibody
      • RING3 antibody
      • RING3 PROTEIN antibody
      • RNF3 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-BRD2 antibody [EPR7642] (ab213347)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-BRD2 antibody [EPR7642] (ab213347)

      ab213347 staining BRD2 in wild-type HAP1 cells (top panel) and BRD2 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213347 at 1/500 dilution (shown in green) and ab195884, Rat anti-Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-BRD2 antibody [EPR7642] (ab213347)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-BRD2 antibody [EPR7642] (ab213347)

      ab213347 staining BRD2 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213347 at 1/1000 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

    • Alexa Fluor® 555 Anti-BRD2 antibody [EPR7642] (ab213347)
      Alexa Fluor® 555 Anti-BRD2 antibody [EPR7642] (ab213347)

    References (1)

    Publishing research using ab213347? Please let us know so that we can cite the reference in this datasheet.

    ab213347 has been referenced in 1 publication.

    • Bagchi RA  et al. HDAC11 suppresses the thermogenic program of adipose tissue via BRD2. JCI Insight 3:N/A (2018). PubMed: 30089714

    Customer reviews and Q&As

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