RecombinantRabMAb

Recombinant Alexa Fluor® 555 Anti-CD3D antibody [EP4426] (ab208514)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-CD3D antibody [EP4426] (ab208514)
  • Alexa Fluor® 555 Anti-CD3D antibody [EP4426] (ab208514)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [EP4426] to CD3D
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

Overview

  • Product name

    Alexa Fluor® 555 Anti-CD3D antibody [EP4426]
    See all CD3D primary antibodies

  • Description

    Alexa Fluor® 555 Rabbit monoclonal [EP4426] to CD3D

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human CD3D aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P04234

  • Positive control

    • ICC/IF: Jurkat cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab208514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min) and 80% methanol (5 min).

Target

  • Function

    The CD3 complex mediates signal transduction.

  • Involvement in disease

    Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.

  • Sequence similarities

    Contains 1 ITAM domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Form

      .

    • Alternative names

      • CD3 antigen delta subunit antibody
      • CD3 delta antibody
      • CD3-delta antibody
      • CD3d antibody
      • CD3d antigen delta polypeptide (TiT3 complex) antibody
      • CD3d molecule antibody
      • CD3d molecule delta (CD3-TCR complex) antibody
      • CD3D_HUMAN antibody
      • IMD19 antibody
      • OKT3 delta chain antibody
      • T cell receptor T3 delta chain antibody
      • T-cell receptor T3 delta chain antibody
      • T-cell surface glycoprotein CD3 delta chain antibody
      • T3D antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-CD3D antibody [EP4426] (ab208514)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-CD3D antibody [EP4426] (ab208514)

      ab208514 staining CD3 in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min) and then incubated in 1%BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab208514 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

       

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

       

      This product also gave a positive signal under the same testing conditions in Jurkat cells fixed with 80% methanol (5 min).

    • Alexa Fluor® 555 Anti-CD3D antibody [EP4426] (ab208514)
      Alexa Fluor® 555 Anti-CD3D antibody [EP4426] (ab208514)

    References (5)

    Publishing research using ab208514? Please let us know so that we can cite the reference in this datasheet.

    ab208514 has been referenced in 5 publications.

    • Eng J  et al. Cyclic Multiplexed-Immunofluorescence (cmIF), a Highly Multiplexed Method for Single-Cell Analysis. Methods Mol Biol 2055:521-562 (2020). PubMed: 31502168
    • Rashid R  et al. Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer. Sci Data 6:323 (2019). beta actin ; Human . PubMed: 31848351
    • Du Z  et al. Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging. Nat Protoc 14:2900-2930 (2019). PubMed: 31534232
    • Lin JR  et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). PubMed: 29993362
    • Jerby-Arnon L  et al. A Cancer Cell Program Promotes T Cell Exclusion and Resistance to Checkpoint Blockade. Cell 175:984-997.e24 (2018). PubMed: 30388455

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