Recombinant Alexa Fluor® 555 Anti-Cdk4 antibody [EPR4513-32-7] (ab215318)
![Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-Cdk4 antibody [EPR4513-32-7] (ab215318)](https://www.abcam.com/ps/products/215/ab215318/Images/ab215318-279937-anti-cdk4-antibody-epr4513-32-7-alexa-fluor-555-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-Cdk4 antibody [EPR4513-32-7] (ab215318)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 555 Rabbit monoclonal [EPR4513-32-7] to Cdk4
- Suitable for: Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 555 Anti-Cdk4 antibody [EPR4513-32-7]
See all Cdk4 primary antibodies -
Description
Alexa Fluor® 555 Rabbit monoclonal [EPR4513-32-7] to Cdk4 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 555. Ex: 555nm, Em: 565nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): T47D cells.
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General notes
This conjugated antibody has been KO validated based on the results obtained with the unconjugated clone: Anti-Cdk4 antibody [EPR4513-32-7] (ab108357).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4513-32-7 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Cdk4 antibody [EPR4513-32-7] (ab108357)
- Alexa Fluor® 488 Anti-Cdk4 antibody [EPR4513-32-7] (ab193966)
- Alexa Fluor® 647 Anti-Cdk4 antibody [EPR4513-32-7] (ab193967)
- HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control (ab193968)
- Anti-Cdk4 antibody [EPR4513-32-7] - BSA and Azide free (ab213216)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/50. |
Target
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Function
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. -
Involvement in disease
Defects in CDK4 are a cause of susceptibility to cutaneous malignant melanoma type 3 (CMM3) [MIM:609048]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B. -
Cellular localization
Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1019 Human
- Omim: 123829 Human
- SwissProt: P11802 Human
- Unigene: 95577 Human
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Alternative names
- Cdk 4 antibody
- cdk4 antibody
- CDK4 protein antibody
see all
Images
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Flow Cytometry (Intracellular) - Alexa Fluor® 555 Anti-Cdk4 antibody [EPR4513-32-7] (ab215318)
Overlay histogram showing T47D cells stained with ab215318 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab215318, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 555 (ab208569) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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![Alexa Fluor® 555 Anti-Cdk4 antibody [EPR4513-32-7] (ab215318)](https://www.abcam.com/ps/products/215/ab215318/Images/ab215318-2-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 555 Anti-Cdk4 antibody [EPR4513-32-7] (ab215318)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab215318 has not yet been referenced specifically in any publications.