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Cell Biology Cell Cycle Kinases/Phosphatases Cdks
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Alexa Fluor® 555 Anti-Cdk4 antibody [EPR4513-32-7] (ab215318)

  • Datasheet
  • Certificate of Compliance
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Flow Cytometry - Alexa Fluor® 555 Anti-Cdk4 antibody [EPR4513-32-7] (ab215318)
  • Alexa Fluor® 555 Anti-Cdk4 antibody [EPR4513-32-7] (ab215318)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [EPR4513-32-7] to Cdk4
  • Suitable for: Flow Cyt
  • Knockout validated
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

You may also be interested in

Protein
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Recombinant Human Cdk4 protein (ab126909)
Primary
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Anti-Cdk4 antibody [EPR4513-32-7] - BSA and Azide free (ab213216)

View more associated products

Overview

  • Product name

    Alexa Fluor® 555 Anti-Cdk4 antibody [EPR4513-32-7]
    See all Cdk4 primary antibodies
  • Description

    Alexa Fluor® 555 Rabbit monoclonal [EPR4513-32-7] to Cdk4
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. within Human Cdk4 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P11802

  • Positive control

    • Flow Cyt: T47D cells.
  • General notes

    This conjugated antibody has been KO validated based on the results obtained with the unconjugated clone: Anti-Cdk4 antibody [EPR4513-32-7] (ab108357).

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4513-32-7
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Cdks
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Cdks
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Cdks

Associated products

  • Alternative Versions

    • Anti-Cdk4 antibody [EPR4513-32-7] (ab108357)
    • Alexa Fluor® 488 Anti-Cdk4 antibody [EPR4513-32-7] (ab193966)
    • Alexa Fluor® 647 Anti-Cdk4 antibody [EPR4513-32-7] (ab193967)
    • HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control (ab193968)
    • Anti-Cdk4 antibody [EPR4513-32-7] - BSA and Azide free (ab213216)
  • Isotype control

    • Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)
  • Recombinant Protein

    • Recombinant Human Cdk4 protein (ab126909)

Applications

Our Abpromise guarantee covers the use of ab215318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.

Target

  • Function

    Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • Involvement in disease

    Defects in CDK4 are a cause of susceptibility to cutaneous malignant melanoma type 3 (CMM3) [MIM:609048]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
  • Cellular localization

    Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
  • Target information above from: UniProt accession P11802 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1019 Human
    • Omim: 123829 Human
    • SwissProt: P11802 Human
    • Unigene: 95577 Human
    • Alternative names

      • Cdk 4 antibody
      • cdk4 antibody
      • CDK4 protein antibody
      • CDK4_HUMAN antibody
      • Cell division kinase 4 antibody
      • Cell division protein kinase 4 antibody
      • CMM 3 antibody
      • CMM3 antibody
      • Crk3 antibody
      • Cyclin dependent kinase 4 antibody
      • Cyclin-dependent kinase 4 antibody
      • Melanoma cutaneous malignant 3 antibody
      • MGC14458 antibody
      • p34 cdk4 antibody
      • PSK J3 antibody
      • PSK-J3 antibody
      see all

    Images

    • Flow Cytometry - Alexa Fluor® 555 Anti-Cdk4 antibody [EPR4513-32-7] (ab215318)
      Flow Cytometry - Alexa Fluor® 555 Anti-Cdk4 antibody [EPR4513-32-7] (ab215318)

      Overlay histogram showing T47D cells stained with ab215318 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab215318, 1/50 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 555 (ab208569) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    • Alexa Fluor® 555 Anti-Cdk4 antibody [EPR4513-32-7] (ab215318)
      Alexa Fluor® 555 Anti-Cdk4 antibody [EPR4513-32-7] (ab215318)

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab215318? Please let us know so that we can cite the reference in this datasheet.

    ab215318 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cdk4 antibody [EPR4513-32-7] (Alexa Fluor® 555)

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (pancreatic adenocarcinoma (PDAC))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate and EDTA
    Permeabilization
    No
    Specification
    pancreatic adenocarcinoma (PDAC)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More

    Jenny Eng

    Verified customer

    Submitted Nov 29 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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