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RecombinantRabMAb

Recombinant Alexa Fluor® 555 Anti-DARPP32 antibody [EP721Y] (ab215663)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-DARPP32 antibody [EP721Y] (ab215663)
  • Alexa Fluor® 555 Anti-DARPP32 antibody [EP721Y] (ab215663)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [EP721Y] to DARPP32
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

You may also be interested in

Primary
Product image
Anti-DARPP32 antibody [EP721Y] - BSA and Azide free (ab239831)

View more associated products

Overview

  • Product name

    Alexa Fluor® 555 Anti-DARPP32 antibody [EP721Y]
    See all DARPP32 primary antibodies
  • Description

    Alexa Fluor® 555 Rabbit monoclonal [EP721Y] to DARPP32
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: U87MG cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP721Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Dopamine Receptors
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Alternative Versions

    • Alexa Fluor® 594 Anti-DARPP32 antibody [EP721Y] (ab214852)
    • Anti-DARPP32 antibody [EP721Y] - BSA and Azide free (ab239831)
    • Anti-DARPP32 antibody [EP721Y] (ab40802)
  • Isotype control

    • Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab215663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in U87MG cells fixed with 100% methanol (5 min)

Notes
ICC/IF
1/100.

This product gave a positive signal in U87MG cells fixed with 100% methanol (5 min)

Target

  • Function

    Inhibitor of protein-phosphatase 1.
  • Sequence similarities

    Belongs to the protein phosphatase inhibitor 1 family.
  • Post-translational
    modifications

    Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
    Phosphorylation of Thr-34 is required for activity.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q9UD71 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 84152 Human
    • Omim: 604399 Human
    • SwissProt: Q9UD71 Human
    • Unigene: 286192 Human
    • Alternative names

      • DARPP 32 antibody
      • DARPP-32 antibody
      • Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
      • Dopamine and cAMP regulated neuronal phosphoprotein antibody
      • Dopamine and cAMP regulated phosphoprotein antibody
      • Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
      • Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
      • Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
      • FLJ20940 antibody
      • IPPD antibody
      • Neuronal phosphoprotein DARPP 32 antibody
      • PPP1R1B antibody
      • PPR1B_HUMAN antibody
      • Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
      • Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
      • Protein phosphatase 1 regulatory subunit 1B antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-DARPP32 antibody [EP721Y] (ab215663)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-DARPP32 antibody [EP721Y] (ab215663)

      ab215663 staining DARPP32 in U87MG cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab215663 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 555 Anti-DARPP32 antibody [EP721Y] (ab215663)
      Alexa Fluor® 555 Anti-DARPP32 antibody [EP721Y] (ab215663)

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab215663? Please let us know so that we can cite the reference in this datasheet.

    ab215663 has not yet been referenced specifically in any publications.

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