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    alexa-fluor-555-irf5-antibody-epr17067-ab210656.pdf

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Immunology Innate Immunity Cytokines Interferons
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RecombinantRabMAb

Recombinant Alexa Fluor® 555 Anti-IRF5 antibody [EPR17067] (ab210656)

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  • Certificate of Compliance
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Flow Cytometry - Alexa Fluor® 555 Anti-IRF5 antibody [EPR17067] (ab210656)
  • Alexa Fluor® 555 Anti-IRF5 antibody [EPR17067] (ab210656)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [EPR17067] to IRF5
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

You may also be interested in

Primary
Product image
Anti-IRF5 antibody [EPR17067] - BSA and Azide free (ab231163)
Conjugation
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Alexa Fluor® 555 Conjugation Kit (Fast) - Lightning-Link® (ab269820)

View more associated products

Overview

  • Product name

    Alexa Fluor® 555 Anti-IRF5 antibody [EPR17067]
    See all IRF5 primary antibodies
  • Description

    Alexa Fluor® 555 Rabbit monoclonal [EPR17067] to IRF5
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Recombinant fragment within Human IRF5 aa 1-200. The exact sequence is proprietary.
    Database link: Q13568-4

  • Positive control

    • Flow Cyt: RAW 264.7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 30% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17067
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol III Transcription
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Helix-Turn-Helix
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Alternative Versions

    • Anti-IRF5 antibody [EPR17067] (ab181553)
    • Alexa Fluor® 488 Anti-IRF5 antibody [EPR17067] (ab208271)
    • Alexa Fluor® 647 Anti-IRF5 antibody [EPR17067] (ab209034)
    • Alexa Fluor® 594 Anti-IRF5 antibody [EPR17067] (ab210457)
    • PE Anti-IRF5 antibody [EPR17067] (ab212239)
    • Anti-IRF5 antibody [EPR17067] - BSA and Azide free (ab231163)
  • Isotype control

    • Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)
  • Recombinant Protein

    • Recombinant Human IRF5 protein (ab114295)

Applications

Our Abpromise guarantee covers the use of ab210656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.

Target

  • Involvement in disease

    Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
    Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
    Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
  • Sequence similarities

    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q13568 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3663 Human
    • Entrez Gene: 27056 Mouse
    • Entrez Gene: 296953 Rat
    • Omim: 607218 Human
    • SwissProt: Q13568 Human
    • SwissProt: P56477 Mouse
    • Unigene: 521181 Human
    • Unigene: 6479 Mouse
    • Unigene: 203787 Rat
    see all
  • Alternative names

    • Interferon regulatory factor 5 antibody
    • Interferon regulatory factor 5 bone marrow variant antibody
    • IRF 5 antibody
    • IRF-5 antibody
    • Irf5 antibody
    • IRF5_HUMAN antibody
    • SLEB10 antibody
    see all

Images

  • Flow Cytometry - Alexa Fluor® 555 Anti-IRF5 antibody [EPR17067] (ab210656)
    Flow Cytometry - Alexa Fluor® 555 Anti-IRF5 antibody [EPR17067] (ab210656)

    Overlay histogram showing RAW 264.7 cells stained with ab210656 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210656, 1/50 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 555 (ab208569) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

  • Alexa Fluor® 555 Anti-IRF5 antibody [EPR17067] (ab210656)
    Alexa Fluor® 555 Anti-IRF5 antibody [EPR17067] (ab210656)

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab210656? Please let us know so that we can cite the reference in this datasheet.

    ab210656 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab210656.
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