Recombinant Alexa Fluor® 555 Anti-Keratin 12/K12 antibody [EPR17882] (ab222916)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 555 Rabbit monoclonal [EPR17882] to Keratin 12/K12
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Overview
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Product name
Alexa Fluor® 555 Anti-Keratin 12/K12 antibody [EPR17882]
See all Keratin 12/K12 primary antibodies -
Description
Alexa Fluor® 555 Rabbit monoclonal [EPR17882] to Keratin 12/K12 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 555. Ex: 555nm, Em: 565nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human Keratin 12/K12 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q99456 -
Positive control
- ICC/IF: Hek293T cells transfected with GFP-tagged Keratin 12/K12.
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General notes
Previously labelled as Keratin 12.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17882 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Keratin 12/K12 antibody [EPR17882] (ab185627)
- Alexa Fluor® 594 Anti-Keratin 12/K12 antibody [EPR17882] (ab219144)
- Alexa Fluor® 488 Anti-Keratin 12/K12 antibody [EPR17882] (ab222116)
- Alexa Fluor® 647 Anti-Keratin 12/K12 antibody [EPR17882] (ab222915)
- Anti-Keratin 12/K12 antibody [EPR17882] - BSA and Azide free (ab240295)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab222916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | 1/100. This product gave a positive signal in Hek293T cells transfected with GFP-tagged Keratin 12 / K12 fixed with 4% formaldehyde (10 min) |
Target
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Function
May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity. -
Tissue specificity
Cornea specific. -
Involvement in disease
Defects in KRT12 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 3859 Human
- Entrez Gene: 268482 Mouse
- Entrez Gene: 360625 Rat
- Omim: 601687 Human
- SwissProt: Q99456 Human
- SwissProt: Q64291 Mouse
- SwissProt: Q6IFW5 Rat
- Unigene: 66739 Human
see all -
Alternative names
- CK-12 antibody
- Cytokeratin-12 antibody
- K12 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Keratin 12/K12 antibody [EPR17882] (ab222916)
ab222916 staining Keratin 12/K12 in Hek293T cells transfected with GFP-tagged Keratin 12/K12 expression construct or GFP-only-tagged expression construct. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.
The cells were then incubated overnight at +4°C with ab222916 at 1/100 dilution (pseudocolored in red) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (pseudocolored in white). Nuclear DNA was labelled with DAPI (shown in blue) and GFP is shown in green.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab222916 has not yet been referenced specifically in any publications.