Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 555 Rabbit monoclonal [EP1422Y] to MelanA
- Suitable for: ICC
- Reacts with: Human
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Related conjugates and formulations
Product nameAlexa Fluor® 555 Anti-MelanA antibody [EP1422Y]
See all MelanA primary antibodies
DescriptionAlexa Fluor® 555 Rabbit monoclonal [EP1422Y] to MelanA
ConjugationAlexa Fluor® 555. Ex: 555nm, Em: 565nm
Tested applicationsSuitable for: ICCmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
- ICC: MeWo cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-MelanA antibody [EP1422Y] - Low endotoxin, Azide free (ab170090)
- Anti-MelanA antibody [EP1422Y] - BSA and Azide free (ab271839)
- Alexa Fluor® 488 Anti-MelanA antibody [EP1422Y] (ab279668)
- Alexa Fluor® 647 Anti-MelanA antibody [EP1422Y] (ab281851)
- Alexa Fluor® 594 Anti-MelanA antibody [EP1422Y] (ab281852)
- Anti-MelanA antibody [EP1422Y] (ab51061)
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab282460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Tissue specificityExpression is restricted to melanoma and melanocyte cell lines and retina.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
- Information by UniProt
- Antigen LB39 AA antibody
- Antigen LB39-AA antibody
- Antigen SK29 AA antibody
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MeWo (human malignant melanoma cell line) cells labelling MelanA with ab282460 at 1/50 dilution (10.0 µg/ml) (Red). Confocal image showing cytoplasmic staining in MeWo cells. ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Green). The Nuclear counterstain was DAPI (Blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab282460 has not yet been referenced specifically in any publications.