RecombinantRabMAb

Recombinant Alexa Fluor® 555 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab274881)

Submit a review Submit a question
Immunocytochemistry - Alexa Fluor® 555 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab274881)

    Key features and details

    • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
    • Alexa Fluor® 555 Rabbit monoclonal [EP1576Y] to S100 beta - Astrocyte Marker
    • Suitable for: ICC
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

    Overview

    • Product name

      Alexa Fluor® 555 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker
      See all S100 beta primary antibodies

    • Description

      Alexa Fluor® 555 Rabbit monoclonal [EP1576Y] to S100 beta - Astrocyte Marker

    • Host species

      Rabbit

    • Conjugation

      Alexa Fluor® 555. Ex: 555nm, Em: 565nm

    • Tested applications

      Suitable for: ICCmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Synthetic peptide within Human S100 beta aa 50 to the C-terminus (C terminal). The exact sequence is proprietary.
      Database link: P04271

    • Positive control

      • ICC: A375 cells.

    • General notes

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free production
      For more information see here.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab274881 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC 1/50.

    This product gave a positive signal in A375 fixed with 4% formaldehyde (10 min).

    Target

    • Function

      Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.

    • Tissue specificity

      Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

    • Sequence similarities

      Belongs to the S-101 family.
      Contains 2 EF-hand domains.

    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • NEF antibody
        • Protein S100 B antibody
        • Protein S100-B antibody
        • S 100 calcium binding protein beta chain antibody
        • S 100 protein beta chain antibody
        • S-100 protein beta chain antibody
        • S-100 protein subunit beta antibody
        • S100 antibody
        • S100 calcium binding protein beta (neural) antibody
        • S100 calcium-binding protein B antibody
        • S100 protein beta chain antibody
        • S100B antibody
        • S100B_HUMAN antibody
        • S100beta antibody
        see all

      Images

      • Immunocytochemistry - Alexa Fluor® 555 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab274881)
        Immunocytochemistry - Alexa Fluor® 555 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab274881)

        Immunofluorescence analysis of A-375 (Human malignant melanoma cell line) cells labeling S100 beta with ab274881. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab274881 at 1/50 dilution (pseudocolored in green) and ab190573, Rabbit monoclonal to alphaTubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab274881? Please let us know so that we can cite the reference in this datasheet.

      ab274881 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab274881.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com