RecombinantRabMAb

Recombinant Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)
  • Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [YE269] to Synaptophysin
  • Suitable for: ICC/IF
  • Reacts with: Rat
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

Overview

  • Product name

    Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269]
    See all Synaptophysin primary antibodies

  • Description

    Alexa Fluor® 555 Rabbit monoclonal [YE269] to Synaptophysin

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Donkey, Cow, Human

  • Immunogen

    Synthetic peptide within Human Synaptophysin aa 250 to the C-terminus (C terminal). The exact sequence is proprietary. Residues in the cytoplasmic domain of human Synaptophysin.
    Database link: P08247
    (Peptide available as ab189853)

  • Positive control

    • ICC/IF: PC12 cells

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab206870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min)

Target

  • Function

    Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.

  • Tissue specificity

    Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.

  • Involvement in disease

    Mental retardation, X-linked, SYP-related

  • Sequence similarities

    Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.

  • Domain

    The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.

  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

  • Cellular localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)

    ab206870 staining Synaptophysin in PC12 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206870 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)
    Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)

References (1)

Publishing research using ab206870? Please let us know so that we can cite the reference in this datasheet.

ab206870 has been referenced in 1 publication.

  • Trias E  et al. Evidence for mast cells contributing to neuromuscular pathology in an inherited model of ALS. JCI Insight 2:N/A (2017). PubMed: 29046475

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