RecombinantRabMAb

Recombinant Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)
  • Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [YE269] to Synaptophysin
  • Suitable for: ICC/IF
  • Reacts with: Rat
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP Unconjugated

Overview

  • Product name

    Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269]
    See all Synaptophysin primary antibodies

  • Description

    Alexa Fluor® 555 Rabbit monoclonal [YE269] to Synaptophysin

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Donkey, Cow, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab189853)

  • Positive control

    • ICC/IF: PC12 cells

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab206870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min)
Notes
ICC/IF
1/100.

This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min)

Target

  • Function

    Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.

  • Tissue specificity

    Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.

  • Involvement in disease

    Mental retardation, X-linked, SYP-related

  • Sequence similarities

    Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.

  • Domain

    The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.

  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

  • Cellular localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)

    ab206870 staining Synaptophysin in PC12 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206870 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)
    Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)

References (3)

Publishing research using ab206870? Please let us know so that we can cite the reference in this datasheet.

ab206870 has been referenced in 3 publications.

  • Sulsenti R  et al. Repurposing of the Antiepileptic Drug Levetiracetam to Restrain Neuroendocrine Prostate Cancer and Inhibit Mast Cell Support to Adenocarcinoma. Front Immunol 12:622001 (2021). PubMed: 33737929
  • O'Grady BJ  et al. Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks. ACS Biomater Sci Eng 6:5811-5822 (2020). PubMed: 33320550
  • Trias E  et al. Evidence for mast cells contributing to neuromuscular pathology in an inherited model of ALS. JCI Insight 2:N/A (2017). PubMed: 29046475

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