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  1. Link

    alexa-fluor-555-tomm20-antibody-epr15581-54-mitochondrial-marker-ab221292.pdf

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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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RecombinantRabMAb

Recombinant Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab221292)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab221292)
  • Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab221292)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [EPR15581-54] to TOMM20 - Mitochondrial Marker
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

You may also be interested in

Protein
Product image
Recombinant Human TOMM20 protein (ab156966)

View more associated products

Overview

  • Product name

    Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker
    See all TOMM20 primary antibodies
  • Description

    Alexa Fluor® 555 Rabbit monoclonal [EPR15581-54] to TOMM20 - Mitochondrial Marker
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human TOMM20 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q15388

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC/IF: HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 30% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15581-54
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial Biogenesis
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Associated products

  • Alternative Versions

    • Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)
    • Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab209606)
    • HRP Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab209951)
    • Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab210047)
    • Alexa Fluor® 594 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab210665)
    • Alexa Fluor® 568 Anti-TOMM20 antibody [EPR15581-54] (ab210841)
    • APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab225341)
    • PE Anti-TOMM20 antibody [EPR15581-54] (ab225342)
    • Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free (ab232589)
  • Isotype control

    • Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)
  • Recombinant Protein

    • Recombinant Human TOMM20 protein (ab156966)

Applications

Our Abpromise guarantee covers the use of ab221292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.
  • Sequence similarities

    Belongs to the Tom20 family.
  • Cellular localization

    Mitochondrion outer membrane.
  • Target information above from: UniProt accession Q15388 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9804 Human
    • Entrez Gene: 67952 Mouse
    • Entrez Gene: 266601 Rat
    • Omim: 601848 Human
    • SwissProt: Q15388 Human
    • SwissProt: Q9DCC8 Mouse
    • SwissProt: Q62760 Rat
    • Unigene: 533192 Human
    • Unigene: 380026 Mouse
    • Unigene: 6932 Mouse
    • Unigene: 2143 Rat
    see all
  • Alternative names

    • KIAA0016 antibody
    • MAS20 antibody
    • MGC117367 antibody
    • Mitochondrial 20 kDa outer membrane protein antibody
    • Mitochondrial import receptor subunit TOM20 homolog antibody
    • MOM19 antibody
    • Outer mitochondrial membrane receptor Tom20 antibody
    • TOM20 antibody
    • TOM20_HUMAN antibody
    • TOMM20 antibody
    • Translocase of outer mitochondrial membrane 20 homolog (yeast) antibody
    • Translocase of outer mitochondrial membrane 20 homolog type II antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab221292)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab221292)

    ab221292 staining TOMM20 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab221292 at 1/1000 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab221292)
    Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab221292)

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab221292? Please let us know so that we can cite the reference in this datasheet.

    ab221292 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (Alexa Fluor® 555)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse Embryonic Fiboblast)
    Permeabilization
    Yes - Digitonin
    Specification
    Mouse Embryonic Fiboblast
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 07 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (Alexa Fluor® 555)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Fibroblast)
    Permeabilization
    Yes - 0.25% Triton X
    Specification
    Fibroblast
    Blocking step
    BSA and 10% goat serum as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Onit Alalouf

    Verified customer

    Submitted Jul 31 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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