RecombinantRabMAb

Recombinant Alexa Fluor® 568 Anti-AMF antibody [EPR11663(B)] (ab213349)

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Flow Cytometry - Alexa Fluor® 568 Anti-AMF antibody [EPR11663(B)] (ab213349)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-AMF antibody [EPR11663(B)] (ab213349)
  • Alexa Fluor® 568 Anti-AMF antibody [EPR11663(B)] (ab213349)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 568 Rabbit monoclonal [EPR11663(B)] to AMF
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm

Overview

  • Product name

    Alexa Fluor® 568 Anti-AMF antibody [EPR11663(B)]
    See all AMF primary antibodies

  • Description

    Alexa Fluor® 568 Rabbit monoclonal [EPR11663(B)] to AMF

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 568. Ex: 578nm, Em: 603nm

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human AMF aa 1-100. The exact sequence is proprietary.
    Database link: P06744

  • Positive control

    • ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells

  • General notes

     This product was previously labelled as Glucose 6 phosphate isomerase

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab213349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/5000.
ICC/IF 1/1000.

This product gave a positive signal in HepG2 cells fixed with 100% methanol (5 min)

Target

  • Function

    Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.

  • Pathway

    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.

  • Involvement in disease

    Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

  • Sequence similarities

    Belongs to the GPI family.

  • Post-translational
    modifications

    Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
    ISGylated.

  • Cellular localization

    Cytoplasm. Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • AMF antibody
      • Aurocrine motility factor antibody
      • Autocrine motility factor antibody
      • DKFZp686C13233 antibody
      • EC 5.3.1.9 antibody
      • G6PI_HUMAN antibody
      • Glucose phosphate isomerase antibody
      • Glucose-6-phosphate isomerase antibody
      • GNPI antibody
      • GPI antibody
      • Gpi1 antibody
      • Hexose monophosphate isomerase antibody
      • Hexosephosphate isomerase antibody
      • Neuroleukin antibody
      • NLK antibody
      • Oxoisomerase antibody
      • PGI antibody
      • PHI antibody
      • Phosphoglucose isomerase antibody
      • Phosphohexomutase antibody
      • Phosphohexose isomerase antibody
      • Phosphosaccharomutase antibody
      • SA 36 antibody
      • SA-36 antibody
      • SA36 antibody
      • Sperm antigen 36 antibody
      see all

    Images

    • Flow Cytometry - Alexa Fluor® 568 Anti-AMF antibody [EPR11663(B)] (ab213349)
      Flow Cytometry - Alexa Fluor® 568 Anti-AMF antibody [EPR11663(B)] (ab213349)

      Overlay histogram showing HepG2 cells stained with ab213349 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab213349, 1/5000 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 568 (ab209613) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-AMF antibody [EPR11663(B)] (ab213349)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-AMF antibody [EPR11663(B)] (ab213349)

      ab213349 staining AFM in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213349 at 1/1000 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 568 Anti-AMF antibody [EPR11663(B)] (ab213349)
      Alexa Fluor® 568 Anti-AMF antibody [EPR11663(B)] (ab213349)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab213349? Please let us know so that we can cite the reference in this datasheet.

    ab213349 has not yet been referenced specifically in any publications.

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