RecombinantRabMAb

Recombinant Alexa Fluor® 568 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab202540)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab202540)
  • Alexa Fluor® 568 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab202540)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 568 Rabbit monoclonal [EPR10823(B)] to Fibrillarin - Nucleolar Marker
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm

Overview

  • Product name

    Alexa Fluor® 568 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker
    See all Fibrillarin primary antibodies

  • Description

    Alexa Fluor® 568 Rabbit monoclonal [EPR10823(B)] to Fibrillarin - Nucleolar Marker

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 568. Ex: 578nm, Em: 603nm

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab202540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
All applications
Mouse
Rat
Application Abreviews Notes
ICC/IF
1/200.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
Notes
ICC/IF
1/200.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function

    S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).

  • Sequence similarities

    Belongs to the methyltransferase superfamily. Fibrillarin family.

  • Post-translational
    modifications

    By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).

  • Cellular localization

    Nucleus, nucleolus. Fibrillar region of the nucleolus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 34 kD nucleolar scleroderma antigen antibody
    • 34 kDa nucleolar scleroderma antigen antibody
    • Fbl antibody
    • FBRL_HUMAN antibody
    • FIB antibody
    • FIB1 antibody
    • FLRN antibody
    • Histone-glutamine methyltransferase antibody
    • Nop1p antibody
    • RNA U3 small nucleolar interacting protein 1 antibody
    • RNU3IP1 antibody
    • rRNA 2' O methyltransferase fibrillarin antibody
    • rRNA 2'-O-methyltransferase fibrillarin antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab202540)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab202540)

    ab202540 staining Fibrillarin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202540 at 1/200 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

  • Alexa Fluor® 568 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab202540)
    Alexa Fluor® 568 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab202540)

References (0)

Publishing research using ab202540? Please let us know so that we can cite the reference in this datasheet.

ab202540 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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