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RecombinantRabMAb

Recombinant Alexa Fluor® 594 Anti-c-Myc antibody [Y69] (ab201775)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-c-Myc antibody [Y69] (ab201775)
  • Alexa Fluor® 594 Anti-c-Myc antibody [Y69] (ab201775)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 594 Rabbit monoclonal [Y69] to c-Myc
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

You may also be interested in

Protein
Recombinant human c-Myc protein (Active) (ab169901)
Knockout
Product image
Human MYC (c-Myc) knockout HEK293T cell line (ab256500)
Conjugation
Alexa Fluor® 594 Conjugation Kit (Fast) - Lightning-Link® (ab269822)

View more associated products

Overview

  • Product name

    Alexa Fluor® 594 Anti-c-Myc antibody [Y69]
    See all c-Myc primary antibodies
  • Description

    Alexa Fluor® 594 Rabbit monoclonal [Y69] to c-Myc
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human c-Myc aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    If you need other conjugated anti-c-myc (Y69) RabMAb antibodies, find our range of products here.

    We also offer a PBS only version of this clone as product ab168727.

    For more information on choosing the right c-Myc antibody for you, please click here.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    Y69
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cancer
    • Tumor biomarkers
    • Oncoproteins

Associated products

  • Alternative Versions

    • Anti-c-Myc antibody [Y69] - BSA and Azide free (ab168727)
    • Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (ab190026)
    • Alexa Fluor® 647 Anti-c-Myc antibody [Y69] (ab190560)
    • Alexa Fluor® 555 Anti-c-Myc antibody [Y69] (ab201780)
    • Alexa Fluor® 568 Anti-c-Myc antibody [Y69] (ab201781)
    • HRP Anti-c-Myc antibody [Y69] (ab205818)
    • FITC Anti-c-Myc antibody [Y69] (ab223913)
    • Anti-c-Myc antibody [Y69] (ab32072)
  • Isotype control

    • Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)
  • Recombinant Protein

    • Recombinant human c-Myc protein (Active) (ab169901)
  • Related Products

    • Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

Applications

Our Abpromise guarantee covers the use of ab201775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
  • Involvement in disease

    Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
    Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications

    Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
    Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
  • Cellular localization

    Nucleus > nucleoplasm. Nucleus > nucleolus.
  • Target information above from: UniProt accession P01106 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4609 Human
    • Omim: 190080 Human
    • SwissProt: P01106 Human
    • Unigene: 202453 Human
    • Form

      c-Myc is also expressed in the cytoplasm.
    • Alternative names

      • AU016757 antibody
      • Avian myelocytomatosis viral oncogene homolog antibody
      • bHLHe39 antibody
      • c Myc antibody
      • Cellular myelocytomatosis oncogene antibody
      • Class E basic helix-loop-helix protein 39 antibody
      • MGC105490 antibody
      • MRTL antibody
      • Myc antibody
      • Myc protein antibody
      • Myc proto oncogene protein antibody
      • Myc proto-oncogene protein antibody
      • myc-related translation/localization regulatory factor antibody
      • MYC_HUMAN antibody
      • Myc2 antibody
      • myca antibody
      • MYCC antibody
      • Myelocytomatosis oncogene a antibody
      • Myelocytomatosis oncogene antibody
      • Niard antibody
      • Nird antibody
      • oncogene c-Myc antibody
      • Oncogene Myc antibody
      • OTTHUMP00000158589 antibody
      • OTTHUMP00000227763 antibody
      • Proto-oncogene c-Myc antibody
      • Protooncogene homologous to myelocytomatosis virus antibody
      • RNCMYC antibody
      • Transcription factor p64 antibody
      • Transcriptional regulator Myc-A antibody
      • V-Myc avian myelocytomatosis viral oncogene homolog antibody
      • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
      • zc-myc antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-c-Myc antibody [Y69] (ab201775)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-c-Myc antibody [Y69] (ab201775)

      ab201775 staining c-Myc in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201775 at a 1/100 dilution (red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 594 Anti-c-Myc antibody [Y69] (ab201775)
      Alexa Fluor® 594 Anti-c-Myc antibody [Y69] (ab201775)

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab201775? Please let us know so that we can cite the reference in this datasheet.

    ab201775 has not yet been referenced specifically in any publications.

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