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Immunology Innate Immunity Chemokines Beta Chemokine Rec. (CCR)
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RecombinantRabMAb

Recombinant Alexa Fluor® 594 Anti-CCR7 antibody [Y59] (ab207016)

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  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-CCR7 antibody [Y59] (ab207016)
  • Alexa Fluor® 594 Anti-CCR7 antibody [Y59] (ab207016)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 594 Rabbit monoclonal [Y59] to CCR7
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

You may also be interested in

Primary
Product image
Anti-CCR7 antibody [Y59] - Low endotoxin, Azide free (ab221209)
Conjugation
Alexa Fluor® 594 Conjugation Kit (Fast) - Lightning-Link® (ab269822)

View more associated products

Overview

  • Product name

    Alexa Fluor® 594 Anti-CCR7 antibody [Y59]
    See all CCR7 primary antibodies
  • Description

    Alexa Fluor® 594 Rabbit monoclonal [Y59] to CCR7
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Jurkat cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y59
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokine Rec. (CCR)
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-CCR7 antibody [Y59] (ab196459)
    • HRP Anti-CCR7 antibody [Y59] (ab196475)
    • Alexa Fluor® 647 Anti-CCR7 antibody [Y59] (ab196640)
    • Alexa Fluor® 555 Anti-CCR7 antibody [Y59] (ab207018)
    • Alexa Fluor® 568 Anti-CCR7 antibody [Y59] (ab207019)
    • Anti-CCR7 antibody [Y59] - Low endotoxin, Azide free (ab221209)
    • Anti-CCR7 antibody [Y59] (ab32527)
  • Isotype control

    • Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)
  • Positive Controls

    • Daudi whole cell lysate (ab3951)
    • K-562 whole cell lysate (ab7911)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
All applications
Mouse
Rat
Rhesus monkey
Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min).

Notes
ICC/IF
1/100.

This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min).

Target

  • Function

    Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
  • Tissue specificity

    Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P32248 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1236 Human
    • Entrez Gene: 12775 Mouse
    • Entrez Gene: 287673 Rat
    • Omim: 600242 Human
    • SwissProt: P32248 Human
    • SwissProt: P47774 Mouse
    • Unigene: 370036 Human
    • Unigene: 2932 Mouse
    • Alternative names

      • BLR 2 antibody
      • BLR2 antibody
      • C C chemokine receptor type 7 antibody
      • C C CKR 7 antibody
      • C-C chemokine receptor type 7 antibody
      • C-C CKR-7 antibody
      • CC chemokine receptor 7 antibody
      • CC chemokine receptor type 7 antibody
      • CC CKR 7 antibody
      • CC-CKR-7 antibody
      • CCCKR7 antibody
      • CCR 7 antibody
      • CCR-7 antibody
      • Ccr7 antibody
      • CCR7_HUMAN antibody
      • CD 197 antibody
      • CD197 antibody
      • CD197 antigen antibody
      • CDw197 antibody
      • Chemokine (C C motif) receptor 7 antibody
      • Chemokine (C C) receptor 7 antibody
      • Chemokine C C motif receptor 7 antibody
      • Chemokine C C receptor 7 antibody
      • Chemokine receptor 7 antibody
      • Chemokine receptor 7-like protein antibody
      • CMKBR7 antibody
      • EBI 1 antibody
      • EBI1 antibody
      • Ebi1h antibody
      • EBV Induced G Protein Coupled Receptor 1 antibody
      • EBV-induced G-protein coupled receptor 1 antibody
      • Epstein Barr virus induced G protein coupled receptor antibody
      • Epstein Barr virus induced gene 1 antibody
      • Epstein-Barr virus-induced G-protein coupled receptor 1 antibody
      • EVI 1 antibody
      • EVI1 antibody
      • Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
      • MGC108519 antibody
      • MIP 3 beta receptor antibody
      • MIP-3 beta receptor antibody
      • MIP3 Beta Receptor antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-CCR7 antibody [Y59] (ab207016)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-CCR7 antibody [Y59] (ab207016)

      ab207016 staining CCR7 in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207016 at 1/100 dilution (shown in orange). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 594 Anti-CCR7 antibody [Y59] (ab207016)
      Alexa Fluor® 594 Anti-CCR7 antibody [Y59] (ab207016)

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab207016? Please let us know so that we can cite the reference in this datasheet.

    ab207016 has not yet been referenced specifically in any publications.

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