RecombinantRabMAb

Recombinant Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab206913)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab206913)
  • Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab206913)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 594 Rabbit monoclonal [EPR4245] to EEA1 - Early Endosome Marker
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Overview

  • Product name

    Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker
    See all EEA1 primary antibodies

  • Description

    Alexa Fluor® 594 Rabbit monoclonal [EPR4245] to EEA1 - Early Endosome Marker

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human EEA1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q15075

  • Positive control

    • ICC/IF: Jurkat cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab206913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.

This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 FYVE-type zinc finger.

  • Domain

    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.

  • Cellular localization

    Cytoplasm. Early endosome membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Early endosome antigen 1 antibody
    • Early endosome antigen 1, 162kD antibody
    • Early endosome associated protein antibody
    • EEA 1 antibody
    • EEA1 antibody
    • EEA1_HUMAN antibody
    • Endosome associated protein p162 antibody
    • Endosome-associated protein p162 antibody
    • MST105 antibody
    • MSTP105 antibody
    • ZFYVE2 antibody
    • Zinc finger FYVE domain containing protein 2 antibody
    • Zinc finger FYVE domain-containing protein 2 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab206913)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab206913)

    ab206913 staining EEA1 in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206913 at 1/1000 dilution (shown in orange). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab206913)
    Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab206913)

References (1)

Publishing research using ab206913? Please let us know so that we can cite the reference in this datasheet.

ab206913 has been referenced in 1 publication.

  • Meng T  et al. In Vitro and In Vivo Inhibition of the Infectivity of Human Enterovirus 71 by a Sulfonated Food Azo Dye, Brilliant Black BN. J Virol 93:N/A (2019). PubMed: 31167919

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