RecombinantRabMAb

Recombinant Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab206913)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab206913)
  • Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab206913)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 594 Rabbit monoclonal [EPR4245] to EEA1 - Early Endosome Marker
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free Unconjugated

Overview

  • Product name

    Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker
    See all EEA1 primary antibodies

  • Description

    Alexa Fluor® 594 Rabbit monoclonal [EPR4245] to EEA1 - Early Endosome Marker

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Jurkat cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab206913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1000.

This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min)
Notes
ICC/IF
1/1000.

This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 FYVE-type zinc finger.

  • Domain

    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.

  • Cellular localization

    Cytoplasm. Early endosome membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Early endosome antigen 1 antibody
      • Early endosome antigen 1, 162kD antibody
      • Early endosome associated protein antibody
      • EEA 1 antibody
      • EEA1 antibody
      • EEA1_HUMAN antibody
      • Endosome associated protein p162 antibody
      • Endosome-associated protein p162 antibody
      • MST105 antibody
      • MSTP105 antibody
      • ZFYVE2 antibody
      • Zinc finger FYVE domain containing protein 2 antibody
      • Zinc finger FYVE domain-containing protein 2 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab206913)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab206913)

      ab206913 staining EEA1 in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206913 at 1/1000 dilution (shown in orange). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab206913)
      Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab206913)

    References (1)

    Publishing research using ab206913? Please let us know so that we can cite the reference in this datasheet.

    ab206913 has been referenced in 1 publication.

    • Meng T  et al. In Vitro and In Vivo Inhibition of the Infectivity of Human Enterovirus 71 by a Sulfonated Food Azo Dye, Brilliant Black BN. J Virol 93:N/A (2019). PubMed: 31167919

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