RecombinantRabMAb

Recombinant Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)
  • Western blot - Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)
  • Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 594 Rabbit monoclonal [EPR3915] to Glucose Transporter GLUT1
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Overview

  • Product name

    Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915]
    See all Glucose Transporter GLUT1 primary antibodies

  • Description

    Alexa Fluor® 594 Rabbit monoclonal [EPR3915] to Glucose Transporter GLUT1

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Glucose Transporter GLUT1 aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: P11166
    (Peptide available as ab202335)

  • Positive control

    • ICC/IF: HepG2 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab206360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.Can be blocked with Glucose Transporter GLUT1 peptide (ab202335).

Target

  • Function

    Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.

  • Tissue specificity

    Expressed at variable levels in many human tissues.

  • Involvement in disease

    Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
    Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.

  • Sequence similarities

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
    • CSE antibody
    • DYT17 antibody
    • DYT18 antibody
    • DYT9 antibody
    • EIG12 antibody
    • erythrocyte/brain antibody
    • Erythrocyte/hepatoma glucose transporter antibody
    • facilitated glucose transporter member 1 antibody
    • Glucose transporter 1 antibody
    • Glucose transporter type 1 antibody
    • Glucose transporter type 1, erythrocyte/brain antibody
    • GLUT antibody
    • GLUT-1 antibody
    • GLUT1 antibody
    • GLUT1DS antibody
    • GLUTB antibody
    • GT1 antibody
    • GTG1 antibody
    • Gtg3 antibody
    • GTR1_HUMAN antibody
    • HepG2 glucose transporter antibody
    • HTLVR antibody
    • Human T cell leukemia virus (I and II) receptor antibody
    • MGC141895 antibody
    • MGC141896 antibody
    • PED antibody
    • RATGTG1 antibody
    • Receptor for HTLV 1 and HTLV 2 antibody
    • SLC2A1 antibody
    • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)

    ab206360 staining Glucose Transporter GLUT1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206360 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5 min).

  • Western blot - Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)
    Western blot - Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)

    Clone EPR3915 has been knock-out validated in its unconjugated form. Please refer to ab115730 datasheet for experimental details.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)

    ab206360 staining Glucose Transporter GLUT1 in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206360 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 4% formaldehyde (10 min).

  • Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)
    Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)

References (0)

Publishing research using ab206360? Please let us know so that we can cite the reference in this datasheet.

ab206360 has not yet been referenced specifically in any publications.

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