Recombinant Alexa Fluor® 594 Anti-MEK1 + MEK2 antibody [EPR16667] (ab208075)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 594 Rabbit monoclonal [EPR16667] to MEK1 + MEK2
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 594 Anti-MEK1 + MEK2 antibody [EPR16667]
See all MEK1 + MEK2 primary antibodies -
Description
Alexa Fluor® 594 Rabbit monoclonal [EPR16667] to MEK1 + MEK2 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 594. Ex: 590nm, Em: 617nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human MEK1 + MEK2 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Also SwissProt ID: P36507
Database link: Q02750 -
Positive control
- ICC/IF: A431 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16667 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-MEK1 + MEK2 antibody [EPR16667] (ab178876)
- Alexa Fluor® 488 Anti-MEK1 + MEK2 antibody [EPR16667] (ab200177)
- HRP Anti-MEK1 + MEK2 antibody [EPR16667] (ab200179)
- Alexa Fluor® 555 Anti-MEK1 + MEK2 antibody [EPR16667] (ab208076)
- Anti-MEK1 + MEK2 antibody [EPR16667] - BSA and Azide free (ab215263)
- APC Anti-MEK1 + MEK2 antibody [EPR16667] (ab225264)
- PE Anti-MEK1 + MEK2 antibody [EPR16667] (ab225265)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab208075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50.
This product gave a positive signal in A431 cells fixed with 4% formaldehyde (10 min) |
Notes |
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ICC/IF
1/50. This product gave a positive signal in A431 cells fixed with 4% formaldehyde (10 min) |
Target
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Function
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. -
Tissue specificity
Widely expressed, with extremely low levels in brain. -
Involvement in disease
Cardiofaciocutaneous syndrome 3 -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The proline-rich region localized between residues 270 and 307 is important for binding to RAF1 and activation of MAP2K1/MEK1. -
Post-translational
modificationsPhosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (RAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK.
Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. -
Cellular localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus. Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis. - Information by UniProt
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Database links
- Entrez Gene: 5604 Human
- Entrez Gene: 5605 Human
- Entrez Gene: 26395 Mouse
- Entrez Gene: 26396 Mouse
- Entrez Gene: 170851 Rat
- Entrez Gene: 58960 Rat
- Omim: 176872 Human
- Omim: 601263 Human
see all -
Alternative names
- AA589381 antibody
- CFC3 antibody
- Dual specificity mitogen-activated protein kinase kinase 1 antibody
see all
Images
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ab208075 staining MEK1 + MEK2 in A431 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208075 at a 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab208075 has not yet been referenced specifically in any publications.