Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

    Key features and details

    • Alexa Fluor® 594 Mouse monoclonal [9E10] to Myc tag
    • Suitable for: ICC/IF
    • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
    • Isotype: IgG1

    Overview

    • Product name

      Alexa Fluor® 594 Anti-Myc tag antibody [9E10]
      See all c-Myc primary antibodies

    • Description

      Alexa Fluor® 594 Mouse monoclonal [9E10] to Myc tag

    • Host species

      Mouse

    • Conjugation

      Alexa Fluor® 594. Ex: 590nm, Em: 617nm

    • Specificity

      This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. A publication by Baker AM et al. 2016 (PMID: 26826706 DOI: 10.1111/his.12939) shows the IHC staining generated by the 9E10 clone does not correlate with c-myc mRNA expression.

    • Tested applications

      Suitable for: ICC/IFmore details

    • Immunogen

      Synthetic peptide within Human Myc tag aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
      Database link: P01106

    • Epitope

      aa 410-419 of human Myc.

    • Positive control

      • ICC/IF: 293T cells transfected with TMEM119 with myc tag

    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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    Properties

    Applications

    Our Abpromise guarantee covers the use of ab223894 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/100.

    This product gave a positive signal in 293T cells transfected with TMEM119 with myc tag fixed with 4% formaldehyde (10 min)

    Target

    • Function

      Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.

    • Involvement in disease

      Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
      Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
      Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.

    • Sequence similarities

      Contains 1 basic helix-loop-helix (bHLH) domain.

    • Post-translational
      modifications

      Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
      Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.

    • Cellular localization

      Nucleus > nucleoplasm. Nucleus > nucleolus.
    • Information by UniProt

    • Form

      c-Myc is also expressed in the cytoplasm.

    • Alternative names

      • AU016757 antibody
      • Avian myelocytomatosis viral oncogene homolog antibody
      • bHLHe39 antibody
      • c Myc antibody
      • Cellular myelocytomatosis oncogene antibody
      • Class E basic helix-loop-helix protein 39 antibody
      • MGC105490 antibody
      • MRTL antibody
      • Myc antibody
      • Myc protein antibody
      • Myc proto oncogene protein antibody
      • Myc proto-oncogene protein antibody
      • myc-related translation/localization regulatory factor antibody
      • MYC_HUMAN antibody
      • Myc2 antibody
      • myca antibody
      • MYCC antibody
      • Myelocytomatosis oncogene a antibody
      • Myelocytomatosis oncogene antibody
      • Niard antibody
      • Nird antibody
      • oncogene c-Myc antibody
      • Oncogene Myc antibody
      • OTTHUMP00000158589 antibody
      • OTTHUMP00000227763 antibody
      • Proto-oncogene c-Myc antibody
      • Protooncogene homologous to myelocytomatosis virus antibody
      • RNCMYC antibody
      • Transcription factor p64 antibody
      • Transcriptional regulator Myc-A antibody
      • V-Myc avian myelocytomatosis viral oncogene homolog antibody
      • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
      • zc-myc antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)

      ab223894 staining Myc-tag (pseudocolored in red) in 293T cells transfected with TMEM119 with myc tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223894 at 1/100 dilution and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue) and Alexa Fluor® 647 conjugated control Myc-tag antibody (different clone) is pseudocolored in white.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab223894? Please let us know so that we can cite the reference in this datasheet.

    ab223894 has not yet been referenced specifically in any publications.

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