RecombinantRabMAb

Recombinant Alexa Fluor® 594 Anti-NME2 antibody [EPR8351] (ab211597)

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Flow Cytometry - Alexa Fluor® 594 Anti-NME2 antibody [EPR8351] (ab211597)
  • Alexa Fluor® 594 Anti-NME2 antibody [EPR8351] (ab211597)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 594 Rabbit monoclonal [EPR8351] to NME2
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Overview

  • Product name

    Alexa Fluor® 594 Anti-NME2 antibody [EPR8351]
    See all NME2 primary antibodies

  • Description

    Alexa Fluor® 594 Rabbit monoclonal [EPR8351] to NME2

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NME2 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P22392

  • Positive control

    • Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab211597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/500.

Target

  • Function

    Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the NDK family.

  • Cellular localization

    Cytoplasm. Nucleus. Isoform 2 is mainly cytoplasmic and isoform 1 and isoform 2 are excluded from the nucleolus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • C myc purine binding transcription factor PUF antibody
    • C myc transcription factor antibody
    • C-myc purine-binding transcription factor PUF antibody
    • epididymis secretory sperm binding protein Li 155an antibody
    • HEL-S-155an antibody
    • Histidine protein kinase NDKB antibody
    • MGC111212 antibody
    • MGC2212 antibody
    • NDK B antibody
    • NDKB antibody
    • NDKB_HUMAN antibody
    • NDP kinase B antibody
    • NDPK B antibody
    • NDPKB antibody
    • NM23 H2 antibody
    • nm23-H2 antibody
    • NM23B antibody
    • NME/NM23 nucleoside diphosphate kinase 2 antibody
    • nme2 antibody
    • Non metastatic cells 2, protein (NM23B) expressed in antibody
    • non-metastatic cells 2, protein (NM23) expressed in antibody
    • Nucleoside diphosphate kinase B antibody
    • Nucleotide diphosphate kinase B antibody
    • PUF antibody
    see all

Images

  • Flow Cytometry - Alexa Fluor® 594 Anti-NME2 antibody [EPR8351] (ab211597)
    Flow Cytometry - Alexa Fluor® 594 Anti-NME2 antibody [EPR8351] (ab211597)
    Overlay histogram showing HeLa cells stained with ab211597 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211597, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 594 (ab208568) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 610/20 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
  • Alexa Fluor® 594 Anti-NME2 antibody [EPR8351] (ab211597)
    Alexa Fluor® 594 Anti-NME2 antibody [EPR8351] (ab211597)

References (0)

Publishing research using ab211597? Please let us know so that we can cite the reference in this datasheet.

ab211597 has not yet been referenced specifically in any publications.

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