Recombinant Alexa Fluor® 594 Anti-NME2 antibody [EPR8351] (ab211597)
![Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-NME2 antibody [EPR8351] (ab211597)](https://www.abcam.com/ps/products/211/ab211597/Images/ab211597-1-ab211597FLOW1.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-NME2 antibody [EPR8351] (ab211597)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 594 Rabbit monoclonal [EPR8351] to NME2
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 594 Anti-NME2 antibody [EPR8351]
See all NME2 primary antibodies -
Description
Alexa Fluor® 594 Rabbit monoclonal [EPR8351] to NME2 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 594. Ex: 590nm, Em: 617nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8351 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/50 - 1/500.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/50 - 1/500. |
Target
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Function
Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the NDK family. -
Cellular localization
Cytoplasm. Nucleus. Isoform 2 is mainly cytoplasmic and isoform 1 and isoform 2 are excluded from the nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 4831 Human
- Entrez Gene: 654364 Human
- Entrez Gene: 18103 Mouse
- Entrez Gene: 83782 Rat
- Omim: 156491 Human
- SwissProt: P22392 Human
- SwissProt: Q01768 Mouse
- SwissProt: P19804 Rat
see all -
Alternative names
- C myc purine binding transcription factor PUF antibody
- C myc transcription factor antibody
- C-myc purine-binding transcription factor PUF antibody
see all
Images
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Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-NME2 antibody [EPR8351] (ab211597)Overlay histogram showing HeLa cells stained with ab211597 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211597, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 594 (ab208568) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 610/20 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions. -
![Alexa Fluor® 594 Anti-NME2 antibody [EPR8351] (ab211597)](https://www.abcam.com/ps/products/211/ab211597/Images/ab211597-2-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 594 Anti-NME2 antibody [EPR8351] (ab211597)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab211597 has not yet been referenced specifically in any publications.