RecombinantRabMAb

Recombinant Alexa Fluor® 594 Anti-PIAS1 antibody [EPR2580(2)] (ab203967)

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Flow Cytometry - Alexa Fluor® 594 Anti-PIAS1 antibody [EPR2580(2)] (ab203967)
  • Alexa Fluor® 594 Anti-PIAS1 antibody [EPR2580(2)] (ab203967)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 594 Rabbit monoclonal [EPR2580(2)] to PIAS1
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Overview

  • Product name

    Alexa Fluor® 594 Anti-PIAS1 antibody [EPR2580(2)]
    See all PIAS1 primary antibodies

  • Description

    Alexa Fluor® 594 Rabbit monoclonal [EPR2580(2)] to PIAS1

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human PIAS1 aa 50-150. The exact sequence is proprietary.
    Database link: O75925

  • Positive control

    • Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab203967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.

Target

  • Function

    Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling.

  • Tissue specificity

    Expressed in numerous tissues with highest level in testis.

  • Pathway

    Protein modification; protein sumoylation.

  • Sequence similarities

    Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.

  • Domain

    The LXXLL motif is a transcriptional coregulator signature.
    The SP-RING-type domain is required for promoting EKLF sumoylation.

  • Post-translational
    modifications

    Sumoylated.
    Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene.

  • Cellular localization

    Nucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AR interacting protein antibody
    • DDXBP1 antibody
    • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody
    • DEAD/H box binding protein 1 antibody
    • DEAD/H box-binding protein 1 antibody
    • E3 SUMO-protein ligase PIAS1 antibody
    • GBP antibody
    • Gu binding protein antibody
    • Gu-binding protein antibody
    • GU/RH-II antibody
    • Pias1 antibody
    • PIAS1_HUMAN antibody
    • Protein inhibitor of activated STAT protein 1 antibody
    • Protein inhibitor of activated STAT, 1 antibody
    • RNA helicase II binding protein antibody
    • RNA helicase II-binding protein antibody
    • Zinc finger, MIZ-type containing 3 antibody
    • ZMIZ3 antibody
    see all

Images

  • Flow Cytometry - Alexa Fluor® 594 Anti-PIAS1 antibody [EPR2580(2)] (ab203967)
    Flow Cytometry - Alexa Fluor® 594 Anti-PIAS1 antibody [EPR2580(2)] (ab203967)
    Overlay histogram showing HeLa cells stained with ab203967 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab203967, 1/50 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 594 (ab208568) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 610/20 bandpass filter.
  • Alexa Fluor® 594 Anti-PIAS1 antibody [EPR2580(2)] (ab203967)
    Alexa Fluor® 594 Anti-PIAS1 antibody [EPR2580(2)] (ab203967)

References (0)

Publishing research using ab203967? Please let us know so that we can cite the reference in this datasheet.

ab203967 has not yet been referenced specifically in any publications.

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