RecombinantRabMAb

Recombinant Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (ab207040)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (ab207040)
  • Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (ab207040)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 594 Rabbit monoclonal [EPNCIR130A] to SATB2
  • Suitable for: ICC/IF
  • Reacts with: Rat
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 APC Carrier Free PE Unconjugated

Overview

  • Product name

    Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A]
    See all SATB2 primary antibodies

  • Description

    Alexa Fluor® 594 Rabbit monoclonal [EPNCIR130A] to SATB2

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human SATB2. The exact sequence is proprietary.
    Database link: Q9UPW6

  • Positive control

    • ICC/IF: PC12 cells

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in PC12 cells fixed with 4% formaldehyde (10 min)
Notes
ICC/IF
1/100.

This product gave a positive signal in PC12 cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.

  • Tissue specificity

    High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.

  • Involvement in disease

    Note=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
    Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
    Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).

  • Sequence similarities

    Belongs to the CUT homeobox family.
    Contains 2 CUT DNA-binding domains.
    Contains 1 homeobox DNA-binding domain.

  • Post-translational
    modifications

    Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.

  • Cellular localization

    Nucleus matrix.
  • Information by UniProt

  • Database links

    • Alternative names

      • DNA binding protein SATB2 antibody
      • DNA-binding protein SATB2 antibody
      • FLJ21474 antibody
      • FLJ32076 antibody
      • GLSS antibody
      • KIAA1034 antibody
      • MGC119474 antibody
      • MGC119477 antibody
      • SATB family member 2 antibody
      • SATB homeobox 2 antibody
      • SATB2 antibody
      • SATB2_HUMAN antibody
      • Special AT rich sequence binding protein 2 antibody
      • Special AT-rich sequence-binding protein 2 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (ab207040)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (ab207040)

      ab207040 staining SATB2 in PC12 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207040 at 1/100 dilution (pseudocolored in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (ab207040)
      Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (ab207040)

    References (0)

    Publishing research using ab207040? Please let us know so that we can cite the reference in this datasheet.

    ab207040 has not yet been referenced specifically in any publications.

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