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    alexa-fluor-594-satb2-antibody-epncir130a-ab207040.pdf

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RecombinantRabMAb

Recombinant Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (ab207040)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (ab207040)
  • Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (ab207040)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 594 Rabbit monoclonal [EPNCIR130A] to SATB2
  • Suitable for: ICC/IF
  • Reacts with: Rat
  • Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm

You may also be interested in

Protein
Product image
Recombinant Human SATB2 protein (ab132405)

View more associated products

Overview

  • Product name

    Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A]
    See all SATB2 primary antibodies
  • Description

    Alexa Fluor® 594 Rabbit monoclonal [EPNCIR130A] to SATB2
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SATB2. The exact sequence is proprietary.
    Database link: Q9UPW6

  • Positive control

    • ICC/IF: PC12 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR130A
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-SATB2 antibody [EPNCIR130A] (ab196316)
    • Alexa Fluor® 647 Anti-SATB2 antibody [EPNCIR130A] (ab196536)
    • Anti-SATB2 antibody [EPNCIR130A] - BSA and Azide free (ab212177)
    • APC Anti-SATB2 antibody [EPNCIR130A] (ab225051)
    • PE Anti-SATB2 antibody [EPNCIR130A] (ab225052)
    • Anti-SATB2 antibody [EPNCIR130A] (ab92446)
  • Isotype control

    • Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)
  • Positive Controls

    • MCF7 nuclear extract lysate (ab14860)
    • Mouse brain tissue lysate - total protein (0 days) (ab7188)
    • Mouse brain tissue lysate - total protein (14 days) (ab7189)
    • Mouse brain tissue lysate - total protein (7 days) (ab7190)
    • SW1353 whole cell lysate (ab7915)
  • Recombinant Protein

    • Recombinant Human SATB2 protein (ab132405)

Applications

Our Abpromise guarantee covers the use of ab207040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in PC12 cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
  • Tissue specificity

    High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
  • Involvement in disease

    Note=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
    Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
    Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).
  • Sequence similarities

    Belongs to the CUT homeobox family.
    Contains 2 CUT DNA-binding domains.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications

    Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
  • Cellular localization

    Nucleus matrix.
  • Target information above from: UniProt accession Q9UPW6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23314 Human
    • Entrez Gene: 212712 Mouse
    • Entrez Gene: 501145 Rat
    • Omim: 608148 Human
    • SwissProt: Q9UPW6 Human
    • SwissProt: Q8VI24 Mouse
    • Unigene: 516617 Human
    • Unigene: 145599 Mouse
    • Unigene: 213596 Rat
    see all
  • Alternative names

    • DNA binding protein SATB2 antibody
    • DNA-binding protein SATB2 antibody
    • FLJ21474 antibody
    • FLJ32076 antibody
    • GLSS antibody
    • KIAA1034 antibody
    • MGC119474 antibody
    • MGC119477 antibody
    • SATB family member 2 antibody
    • SATB homeobox 2 antibody
    • SATB2 antibody
    • SATB2_HUMAN antibody
    • Special AT rich sequence binding protein 2 antibody
    • Special AT-rich sequence-binding protein 2 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (ab207040)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (ab207040)

    ab207040 staining SATB2 in PC12 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207040 at 1/100 dilution (pseudocolored in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (ab207040)
    Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (ab207040)

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab207040? Please let us know so that we can cite the reference in this datasheet.

    ab207040 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab207040.
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