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    alexa-fluor-647-acadmmcad-antibody-3b7bh7-ab198099.pdf

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Signal Transduction Metabolism Mitochondrial
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Alexa Fluor® 647 Anti-ACADM/MCAD antibody [3B7BH7] (ab198099)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ACADM/MCAD antibody [3B7BH7] (ab198099)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [3B7BH7] to ACADM/MCAD
    • Suitable for: ICC/IF
    • Reacts with: Mouse, Rat, Cow, Human
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant Human ACADM/MCAD protein (ab99329)

    View more associated products

    Overview

    • Product name

      Alexa Fluor® 647 Anti-ACADM/MCAD antibody [3B7BH7]
      See all ACADM/MCAD primary antibodies
    • Description

      Alexa Fluor® 647 Mouse monoclonal [3B7BH7] to ACADM/MCAD
    • Host species

      Mouse
    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Cow, Human
    • Immunogen

      Full length protein corresponding to ACADM/MCAD.

    • Positive control

      • ICC/IF: HeLa cells
    • General notes

      Previously labelled as ACADM. 

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 30% Glycerol, 1% BSA, PBS
    • Concentration information loading...
    • Purity

      Ammonium Sulphate Precipitation
    • Purification notes

      Produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
    • Clonality

      Monoclonal
    • Clone number

      3B7BH7
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Signal Transduction
      • Metabolism
      • Mitochondrial
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cardiovascular
      • Lipids / Lipoproteins
      • Fatty Acids
      • Metabolism
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Fatty acid oxidation

    Associated products

    • Alternative Versions

      • Anti-ACADM/MCAD antibody [3B7BH7] (ab110296)
      • Alexa Fluor® 488 Anti-ACADM/MCAD antibody [3B7BH7] (ab198078)
    • Positive Controls

      • HeLa membrane extract lysate (ab29547)
    • Recombinant Protein

      • Recombinant Human ACADM/MCAD protein (ab99329)
    • Related Products

      • Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

    Applications

    Our Abpromise guarantee covers the use of ab198099 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/500.

    This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

    Target

    • Function

      This enzyme is specific for acyl chain lengths of 4 to 16.
    • Pathway

      Lipid metabolism; mitochondrial fatty acid beta-oxidation.
    • Involvement in disease

      Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
    • Sequence similarities

      Belongs to the acyl-CoA dehydrogenase family.
    • Cellular localization

      Mitochondrion matrix.
    • Target information above from: UniProt accession P11310 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 505968 Cow
      • Entrez Gene: 34 Human
      • Entrez Gene: 11364 Mouse
      • Entrez Gene: 24158 Rat
      • Omim: 607008 Human
      • SwissProt: Q3SZB4 Cow
      • SwissProt: P11310 Human
      • SwissProt: P45952 Mouse
      • SwissProt: P08503 Rat
      • Unigene: 445040 Human
      • Unigene: 10530 Mouse
      • Unigene: 6302 Rat
      see all
    • Alternative names

      • ACAD 1 antibody
      • ACAD1 antibody
      • Acadm antibody
      • ACADM_HUMAN antibody
      • Acyl coenzyme A dehydrogenase antibody
      • Acyl coenzyme A dehydrogenase C 4 to C 12 straight chain antibody
      • FLJ18227 antibody
      • FLJ93013 antibody
      • FLJ99884 antibody
      • MCAD antibody
      • MCADH antibody
      • Medium chain acyl CoA dehydrogenase antibody
      • Medium chain fatty acyl CoA dehydrogenase antibody
      • Medium chain specific acyl CoA dehydrogenase antibody
      • Medium chain specific acyl CoA dehydrogenase mitochondrial antibody
      • Medium-chain specific acyl-CoA dehydrogenase antibody
      • mitochondrial antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ACADM/MCAD antibody [3B7BH7] (ab198099)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ACADM/MCAD antibody [3B7BH7] (ab198099)

      ab198099 staining ACADM/MCAD in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198099 at a 1/500 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab198099? Please let us know so that we can cite the reference in this datasheet.

    ab198099 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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