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Alexa Fluor® 647 Anti-Aconitase 2 antibody [6F12BD9] (ab198050)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Aconitase 2 antibody [6F12BD9] (ab198050)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [6F12BD9] to Aconitase 2
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant Human Aconitase 2 protein (ab114378)
    Conjugation
    Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (ab269823)

    View more associated products

    Overview

    • Product name

      Alexa Fluor® 647 Anti-Aconitase 2 antibody [6F12BD9]
      See all Aconitase 2 primary antibodies
    • Description

      Alexa Fluor® 647 Mouse monoclonal [6F12BD9] to Aconitase 2
    • Host species

      Mouse
    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Tested Applications & Species

      Application Species
      ICC/IF
      Human
      See all applications and species data
    • Positive control

      • ICC/IF: HeLa cells.
    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
    • Concentration information loading...
    • Purity

      Affinity purified
    • Clonality

      Monoclonal
    • Clone number

      6F12BD9
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Tags & Cell Markers
      • Subcellular Markers
      • Organelles
      • Mitochondria
      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Signal Transduction
      • Metabolism
      • Mitochondrial
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism

    Associated products

    • Alternative Versions

      • Anti-Aconitase 2 antibody [6F12BD9] (ab110321)
      • Alexa Fluor® 488 Anti-Aconitase 2 antibody [6F12BD9] (ab198049)
    • Recombinant Protein

      • Recombinant Human Aconitase 2 protein (ab114378)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab198050 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    ICC/IF
    Human
    All applications
    Mouse
    Rat
    Application Abreviews Notes
    ICC/IF
    1/100.

    This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).

    Notes
    ICC/IF
    1/100.

    This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).

    Target

    • Function

      Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
    • Pathway

      Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
    • Sequence similarities

      Belongs to the aconitase/IPM isomerase family.
    • Cellular localization

      Mitochondrion.
    • Target information above from: UniProt accession Q99798 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 50 Human
      • Entrez Gene: 11429 Mouse
      • Entrez Gene: 79250 Rat
      • Omim: 100850 Human
      • SwissProt: Q99798 Human
      • SwissProt: Q99KI0 Mouse
      • SwissProt: Q9ER34 Rat
      • Unigene: 643610 Human
      • Unigene: 154581 Mouse
      • Unigene: 43737 Rat
      see all
    • Alternative names

      • ACO 2 antibody
      • ACO2 antibody
      • ACON_HUMAN antibody
      • aconitase 2 antibody
      • Aconitase 2 mitochondrial antibody
      • Aconitase antibody
      • Aconitase2 antibody
      • Aconitate hydratase antibody
      • Aconitate hydratase mitochondrial antibody
      • ACONM antibody
      • Citrate hydro lyase antibody
      • Citrate hydro-lyase antibody
      • ICRD antibody
      • mitochondrial antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Aconitase 2 antibody [6F12BD9] (ab198050)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Aconitase 2 antibody [6F12BD9] (ab198050)

      ab198050 staining Aconitase 2 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198050 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab198050? Please let us know so that we can cite the reference in this datasheet.

    ab198050 has not yet been referenced specifically in any publications.

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