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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cell Adhesion Molecules Endothelial
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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab214259)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab214259)
  • Alexa Fluor® 647 Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab214259)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR11449(B)] to ADRM1/ARM-1
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

You may also be interested in

Protein
Product image
Recombinant Human ADRM1/ARM-1 protein (ab124570)
Primary
Product image
Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free (ab249293)

View more associated products

Overview

  • Product name

    Alexa Fluor® 647 Anti-ADRM1/ARM-1 antibody [EPR11449(B)]
    See all ADRM1/ARM-1 primary antibodies
  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR11449(B)] to ADRM1/ARM-1
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells
  • General notes

     This product was previously labelled as ADRM1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11449(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial

Associated products

  • Alternative Versions

    • Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab157185)
    • PE Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab211702)
    • Anti-ADRM1/ARM-1 antibody [EPR11449(B)] - BSA and Azide free (ab249293)
  • Isotype control

    • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human ADRM1/ARM-1 protein (ab124570)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab214259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)

Notes
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity.
  • Sequence similarities

    Belongs to the ADRM1 family.
    Contains 1 PH domain.
  • Domain

    The PH domain mediates interactions with PSMD1 and ubiquitin. Preferential binding to the proximal subunit of K48-linked diubiquitin allows UCHL5 access to the distal subunit.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession Q16186 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 11047 Human
    • Omim: 610650 Human
    • SwissProt: Q16186 Human
    • Unigene: 90107 Human
    • Alternative names

      • 110 kDa cell membrane glycoprotein antibody
      • adhesion regulating molecule 1 antibody
      • Adhesion-regulating molecule 1 antibody
      • ADRM 1 antibody
      • Adrm1 antibody
      • ADRM1_HUMAN antibody
      • ARM 1 antibody
      • ARM-1 antibody
      • ARM1 antibody
      • Gp110 antibody
      • hRpn13 antibody
      • M(r) 110,000 surface antigen antibody
      • Proteasomal ubiquitin receptor ADRM1 antibody
      • proteasome regulatory particle non ATPase 13 antibody
      • Proteasome regulatory particle non-ATPase 13 antibody
      • Regulatory particle non ATPase 13 antibody
      • Rpn13 antibody
      • Rpn13 homolog antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab214259)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab214259)

      Ab214259 staining ADRM1/ARM-1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), and then incubated in 1%BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab214259 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

       

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab214259)
      Alexa Fluor® 647 Anti-ADRM1/ARM-1 antibody [EPR11449(B)] (ab214259)

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab214259? Please let us know so that we can cite the reference in this datasheet.

    ab214259 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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