For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    alexa-fluor-647-aif-antibody-7f7ab10-ab198055.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Apoptosis Nucleus Chromatin Condensation
Share by email

Alexa Fluor® 647 Anti-AIF antibody [7F7AB10] (ab198055)

  • Datasheet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-AIF antibody [7F7AB10] (ab198055)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [7F7AB10] to AIF
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant Human AIF protein (ab177656)
    Knockout
    Product image
    Human AIFM1 (AIF) knockout HEK293T cell line (ab266347)

    View more associated products

    Overview

    • Product name

      Alexa Fluor® 647 Anti-AIF antibody [7F7AB10]
      See all AIF primary antibodies
    • Description

      Alexa Fluor® 647 Mouse monoclonal [7F7AB10] to AIF
    • Host species

      Mouse
    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue, cells or virus corresponding to Human AIF.

    • Positive control

      • ICC/IF: HeLa cells.
    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 30% Glycerol, 1% BSA
    • Concentration information loading...
    • Purity

      Affinity purified
    • Purification notes

      ab110327 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
    • Clonality

      Monoclonal
    • Clone number

      7F7AB10
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Cell Biology
      • Apoptosis
      • Nucleus
      • Chromatin Condensation
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Apoptosis
      • Nuclear
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Chromatin condensation
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Oxidative stress
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Apoptosis
      • Cancer
      • Cell Death
      • Apoptosis
      • Nucleus
      • Chromatin condensation
      • Cancer
      • Cell Death
      • Apoptosis
      • Metabolism

    Associated products

    • Alternative Versions

      • Anti-AIF antibody [7F7AB10] (ab110327)
      • Alexa Fluor® 488 Anti-AIF antibody [7F7AB10] (ab198313)
    • Recombinant Protein

      • Recombinant Human AIF protein (ab177656)

    Applications

    Our Abpromise guarantee covers the use of ab198055 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/50.

    This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

    Target

    • Function

      Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
    • Involvement in disease

      Defects in AIFM1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6) [MIM:300816]. It is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.
    • Sequence similarities

      Belongs to the FAD-dependent oxidoreductase family.
    • Post-translational
      modifications

      Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
    • Cellular localization

      Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.
    • Target information above from: UniProt accession O95831 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 51060 Human
      • Entrez Gene: 9131 Human
      • Omim: 300169 Human
      • SwissProt: O95831 Human
      • Unigene: 424932 Human
      • Unigene: 476033 Human
      • Alternative names

        • AIFM1 antibody
        • AIFM1_HUMAN antibody
        • Apoptosis inducing factor 1, mitochondrial antibody
        • Apoptosis inducing factor antibody
        • Apoptosis inducing factor, mitochondrion associated, 1 antibody
        • Apoptosis-inducing factor 1 antibody
        • CMTX4 antibody
        • COWCK antibody
        • COXPD6 antibody
        • Harlequin antibody
        • Hq antibody
        • mAIF antibody
        • MGC111425 antibody
        • MGC5706 antibody
        • mitochondrial antibody
        • Neuropathy, axonal motor-sensory, with deafness and mental retardation antibody
        • neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) antibody
        • PDCD 8 antibody
        • PDCD8 antibody
        • Programmed cell death 8 (apoptosis inducing factor) antibody
        • Programmed cell death 8 antibody
        • Programmed cell death 8 isoform 1 antibody
        • Programmed cell death 8 isoform 2 antibody
        • Programmed cell death 8 isoform 3 antibody
        • Programmed cell death protein 8 antibody
        • Programmed cell death protein 8 mitochondrial antibody
        • Programmed cell death protein 8 mitochondrial precursor antibody
        • Programmed cell death protein 8 mitochondrial precursor antibody
        • Striatal apoptosis inducing factor antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-AIF antibody [7F7AB10] (ab198055)
        Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-AIF antibody [7F7AB10] (ab198055)

        ab198055 staining AIF in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198055 at a 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

        This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab198055? Please let us know so that we can cite the reference in this datasheet.

      ab198055 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab198055.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.