Alexa Fluor® 647 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab196695)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab196695)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG1

    Overview

    • Product name

      Alexa Fluor® 647 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]
      See all alpha 1 Sodium Potassium ATPase primary antibodies

    • Description

      Alexa Fluor® 647 Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase

    • Host species

      Mouse

    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Full length native protein (purified) corresponding to Rabbit alpha 1 Sodium Potassium ATPase.

    • Positive control

      • ICC/IF: HEK293 cells.

    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

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      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 30% Glycerol, 1% BSA
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Monoclonal

    • Clone number

      464.6

    • Isotype

      IgG1

    • Light chain type

      kappa

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab196695 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/50.

    This product gave a positive signal in Hek293 cells fixed with 100% methanol (5 min)

    Target

    • Function

      This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

    • Sequence similarities

      Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

    • Post-translational
      modifications

      Phosphorylation on Tyr-10 modulates pumping activity.

    • Cellular localization

      Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt

    • Database links

      • Alternative names

        • A1A1 antibody
        • AT1A1 antibody
        • AT1A1_HUMAN antibody
        • ATP1A1 antibody
        • Atpa-1 antibody
        • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
        • ATPase Na+/K+ transporting subunit alpha 1 antibody
        • BC010319 antibody
        • EC 3.6.3.9 antibody
        • MGC3285 antibody
        • MGC38419 antibody
        • MGC51750 antibody
        • Na K ATPase alpha A catalytic polypeptide antibody
        • Na K ATPase catalytic subunit alpha A protein antibody
        • Na(+)/K(+) ATPase 1 antibody
        • Na(+)/K(+) ATPase alpha-1 subunit antibody
        • Na+, K+ ATPase alpha subunit antibody
        • Na+/K+ ATPase alpha 1 subunit antibody
        • Na+/K+ ATPase 1 antibody
        • Na,K ATPase alpha 1 subunit antibody
        • Nkaa1b antibody
        • Sodium potassium ATPase alpha 1 polypeptide antibody
        • Sodium pump 1 antibody
        • Sodium pump subunit alpha-1 antibody
        • sodium-potassium ATPase catalytic subunit alpha-1 antibody
        • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab196695)
        Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab196695)

        ab196695 staining alpha 1 Sodium Potassium ATPase in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196695 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      References (0)

      Publishing research using ab196695? Please let us know so that we can cite the reference in this datasheet.

      ab196695 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (Alexa Fluor® 647)

       Average
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (Kidney)
      Permeabilization
      No
      Specification
      Kidney
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 28 2016

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