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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family
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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-ARF1 + ARF3 antibody [EPR443] (ab207645)

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  • SDS
  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ARF1 + ARF3 antibody [EPR443] (ab207645)
  • Alexa Fluor® 647 Anti-ARF1 + ARF3 antibody [EPR443] (ab207645)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR443] to ARF1 + ARF3
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

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Conjugation
Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (ab269823)

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Overview

  • Product name

    Alexa Fluor® 647 Anti-ARF1 + ARF3 antibody [EPR443]
  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR443] to ARF1 + ARF3
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: CaCo2 cells
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR443
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Signal Transduction
    • Protein Trafficking
    • ER Proteins

Associated products

  • Alternative Versions

    • Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
    • Anti-ARF1+ARF3 antibody [EPR443] - BSA and Azide free (ab247639)
  • Isotype control

    • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
All applications
Mouse
Rat
Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in CaCo2 cells fixed with 100% methanol (5 min)

Notes
ICC/IF
1/100.

This product gave a positive signal in CaCo2 cells fixed with 100% methanol (5 min)

Target

  • Cellular localization

    ARF1: Golgi apparatus. Cytoplasm > perinuclear region. ARF3: Golgi apparatus. Cytoplasm > perinuclear region.
  • Database links

    • Entrez Gene: 375 Human
    • Entrez Gene: 377 Human
    • Entrez Gene: 11840 Mouse
    • Entrez Gene: 11842 Mouse
    • Entrez Gene: 140940 Rat
    • Entrez Gene: 64310 Rat
    • Omim: 103180 Human
    • Omim: 103190 Human
    • SwissProt: P61204 Human
    • SwissProt: P84077 Human
    • SwissProt: P61205 Mouse
    • SwissProt: P84078 Mouse
    • SwissProt: P61206 Rat
    • SwissProt: P84079 Rat
    • Unigene: 119177 Human
    • Unigene: 286221 Human
    • Unigene: 221298 Mouse
    • Unigene: 371546 Mouse
    • Unigene: 6836 Mouse
    • Unigene: 106440 Rat
    • Unigene: 93735 Rat
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ARF1 + ARF3 antibody [EPR443] (ab207645)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ARF1 + ARF3 antibody [EPR443] (ab207645)

    ab207645 staining ARF1 + ARF3 in CaCo2 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207645 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 647 Anti-ARF1 + ARF3 antibody [EPR443] (ab207645)
    Alexa Fluor® 647 Anti-ARF1 + ARF3 antibody [EPR443] (ab207645)

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab207645? Please let us know so that we can cite the reference in this datasheet.

ab207645 has not yet been referenced specifically in any publications.

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