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    alexa-fluor-647-atp-citrate-lyase-antibody-ep704y-ab205430.pdf

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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y] (ab205430)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y] (ab205430)
  • Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y] (ab205430)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EP704Y] to ATP citrate lyase
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

You may also be interested in

Primary
Product image
Alexa Fluor® 488 Anti-ATP citrate lyase antibody [EP704Y] (ab205429)
Protein
Product image
Recombinant Human ATP citrate lyase protein (His tag) (ab235007)

View more associated products

Overview

  • Product name

    Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y]
    See all ATP citrate lyase primary antibodies
  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EP704Y] to ATP citrate lyase
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Common marmoset
  • Immunogen

    Synthetic peptide within Human ATP citrate lyase aa 1050 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP704Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-ATP citrate lyase antibody [EP704Y] (ab205429)
    • PE Anti-ATP citrate lyase antibody [EP704Y] (ab209750)
    • Anti-ATP citrate lyase antibody [EP704Y] - BSA and Azide free (ab227996)
    • Anti-ATP citrate lyase antibody [EP704Y] (ab40793)
  • Isotype control

    • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)
  • Recombinant Protein

    • Recombinant Human ATP citrate lyase protein (His tag) (ab235007)
  • Related Products

    • Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

Applications

Our Abpromise guarantee covers the use of ab205430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/167.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).

Target

  • Function

    ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
  • Sequence similarities

    In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
    In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
    Contains 1 ATP-grasp domain.
  • Post-translational
    modifications

    ISGylated.
    Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
    Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P53396 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 47 Human
    • Entrez Gene: 104112 Mouse
    • Entrez Gene: 24159 Rat
    • Omim: 108728 Human
    • SwissProt: P53396 Human
    • SwissProt: Q91V92 Mouse
    • SwissProt: P16638 Rat
    • Unigene: 387567 Human
    • Unigene: 282039 Mouse
    • Unigene: 29771 Rat
    see all
  • Alternative names

    • ACL antibody
    • Acly antibody
    • ACLY_HUMAN antibody
    • ATP citrate (pro-S) lyase antibody
    • ATP citrate lyase antibody
    • ATP citrate synthase antibody
    • ATP-citrate (pro-S-)-lyase antibody
    • ATP-citrate synthase antibody
    • ATPcitrate synthase antibody
    • ATPCL antibody
    • Citrate cleavage enzyme antibody
    • CLATP antibody
    • OTTHUMP00000164773 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y] (ab205430)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y] (ab205430)

    ab205430 staining ATP citrate lyase in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205430 at 1/167 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y] (ab205430)
    Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y] (ab205430)

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab205430? Please let us know so that we can cite the reference in this datasheet.

    ab205430 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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