RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y] (ab205430)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y] (ab205430)
  • Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y] (ab205430)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EP704Y] to ATP citrate lyase
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free PE Unconjugated

Overview

  • Product name

    Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y]
    See all ATP citrate lyase primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EP704Y] to ATP citrate lyase

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Common marmoset

  • Immunogen

    Synthetic peptide within Human ATP citrate lyase aa 1050 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab205430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/167.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).
Notes
ICC/IF
1/167.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).

Target

  • Function

    ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.

  • Sequence similarities

    In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
    In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
    Contains 1 ATP-grasp domain.

  • Post-translational
    modifications

    ISGylated.
    Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
    Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ACL antibody
    • Acly antibody
    • ACLY_HUMAN antibody
    • ATP citrate (pro-S) lyase antibody
    • ATP citrate lyase antibody
    • ATP citrate synthase antibody
    • ATP-citrate (pro-S-)-lyase antibody
    • ATP-citrate synthase antibody
    • ATPcitrate synthase antibody
    • ATPCL antibody
    • Citrate cleavage enzyme antibody
    • CLATP antibody
    • OTTHUMP00000164773 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y] (ab205430)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y] (ab205430)

    ab205430 staining ATP citrate lyase in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205430 at 1/167 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y] (ab205430)
    Alexa Fluor® 647 Anti-ATP citrate lyase antibody [EP704Y] (ab205430)

References (0)

Publishing research using ab205430? Please let us know so that we can cite the reference in this datasheet.

ab205430 has not yet been referenced specifically in any publications.

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