Alexa Fluor® 647 Anti-ATP5H antibody [10G5AB9] (ab198403)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATP5H antibody [10G5AB9] (ab198403)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [10G5AB9] to ATP5H
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG1

    Overview

    • Product name

      Alexa Fluor® 647 Anti-ATP5H antibody [10G5AB9]
      See all ATP5H primary antibodies

    • Description

      Alexa Fluor® 647 Mouse monoclonal [10G5AB9] to ATP5H

    • Host species

      Mouse

    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Full length native protein (purified) corresponding to Cow ATP5H. Purified bovine OXPHOS complexes.

    • Positive control

      • ICC/IF: HeLa cells.

    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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    Properties

    Applications

    Our Abpromise guarantee covers the use of ab198403 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/100.

    Target

    • Function

      Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

    • Sequence similarities

      Belongs to the ATPase d subunit family.

    • Cellular localization

      Mitochondrion. Mitochondrion inner membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • ATP synthase D chain mitochondrial antibody
        • ATP synthase H+ transporting mitochondrial F1F0 subunit antibody
        • ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody
        • ATP synthase subunit d antibody
        • ATP synthase subunit d, mitochondrial antibody
        • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody
        • ATP5H antibody
        • ATP5H_HUMAN antibody
        • ATP5JD antibody
        • ATPase subunit d antibody
        • ATPQ antibody
        • mitochondrial antibody
        • My032 protein antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATP5H antibody [10G5AB9] (ab198403)
        Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATP5H antibody [10G5AB9] (ab198403)

        ab198403 staining ATP5H in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab198403 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      References (0)

      Publishing research using ab198403? Please let us know so that we can cite the reference in this datasheet.

      ab198403 has not yet been referenced specifically in any publications.

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