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Alexa Fluor® 647 Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7] (ab198077)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7] (ab198077)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [5E2D7] to ATPase Inhibitory Factor 1/IF1
    • Suitable for: ICC/IF
    • Reacts with: Mouse, Rat, Cow, Human
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant Human ATPase Inhibitory Factor 1/IF1 protein (ab130030)
    Primary
    Product image
    Alexa Fluor® 488 Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7] (ab198075)

    View more associated products

    Overview

    • Product name

      Alexa Fluor® 647 Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7]
      See all ATPase Inhibitory Factor 1/IF1 primary antibodies
    • Description

      Alexa Fluor® 647 Mouse monoclonal [5E2D7] to ATPase Inhibitory Factor 1/IF1
    • Host species

      Mouse
    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Cow, Human
    • Immunogen

      Recombinant full length protein corresponding to Rat ATPase Inhibitory Factor 1/IF1.

    • Positive control

      • ICC/IF: HepG2 cells.
    • General notes

      Previously labelled as ATPase Inhibitory Factor 1. 

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 1% BSA, 30% Glycerol
    • Concentration information loading...
    • Purity

      Affinity purified
    • Clonality

      Monoclonal
    • Clone number

      5E2D7
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Tags & Cell Markers
      • Subcellular Markers
      • Organelles
      • Mitochondria
      • Signal Transduction
      • Metabolism
      • Mitochondrial
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Oxidative phosphorylation
      • Complex V

    Associated products

    • Alternative Versions

      • Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7] (ab110277)
      • Alexa Fluor® 488 Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7] (ab198075)
    • Isotype control

      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    • Recombinant Protein

      • Recombinant Human ATPase Inhibitory Factor 1/IF1 protein (ab130030)

    Applications

    Our Abpromise guarantee covers the use of ab198077 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/100.

    This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min).

    Target

    • Function

      Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix.
    • Sequence similarities

      Belongs to the ATPase inhibitor family.
    • Post-translational
      modifications

      Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.
    • Cellular localization

      Mitochondrion.
    • Target information above from: UniProt accession Q9UII2 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 327699 Cow
      • Entrez Gene: 93974 Human
      • Entrez Gene: 11983 Mouse
      • Entrez Gene: 25392 Rat
      • SwissProt: P01096 Cow
      • SwissProt: Q9UII2 Human
      • SwissProt: O35143 Mouse
      • SwissProt: Q03344 Rat
      • Unigene: 726089 Human
      • Unigene: 2171 Mouse
      • Unigene: 1658 Rat
      see all
    • Alternative names

      • ATIF1_HUMAN antibody
      • ATP synthase inhibitor protein antibody
      • ATPase inhibitor antibody
      • ATPase inhibitor mitochondrial antibody
      • ATPase inhibitor protein antibody
      • ATPase inhibitory factor 1 antibody
      • ATPI antibody
      • ATPIF 1 antibody
      • Atpif1 antibody
      • ATPIP antibody
      • IF(1) antibody
      • IF1 antibody
      • Inhibitor of F(1)F(o)-ATPase antibody
      • IP antibody
      • MGC1167 antibody
      • MGC8898 antibody
      • mitochondrial antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7] (ab198077)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7] (ab198077)

      ab198077 staining ATPase Inhibitory Factor 1/IF1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198077 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab198077? Please let us know so that we can cite the reference in this datasheet.

    ab198077 has not yet been referenced specifically in any publications.

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